eng
Iran University of Medical Sciences
Basic and Clinical Neuroscience
2008-126X
2228-7442
2021-05
12
3
301
308
article
The Effect of 6-gingerol on Growth Factors and Apoptosis Indices in Rats Exposed to Gold Nanoparticles
Ghasem Majdi Yazdi
majdi131@yahoo.com
1
Gholamhasan Vaezi
gh.vaezi@yahoo.com
2
Vida Hojati
vida.hojati@gmail.com
3
Mohammad Mohammad-Zadeh
mohammadzadehmh@mums.ac.ir
4
Department of Biology, Faculty of Basic Sciences, Damghan Branch, Islamic Azad University, Damghan, Iran.
Department of Biology, Faculty of Basic Sciences, Damghan Branch, Islamic Azad University, Damghan, Iran.
Department of Biology, Faculty of Basic Sciences, Damghan Branch, Islamic Azad University, Damghan, Iran.
Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Introduction: Research has shown that gold nanoparticles (AuNPs) can damage the physiological processes of brain tissue. Given the antioxidant properties of Gingerol (GING), this study aimed to determine the protective effect of 6-gingerol on hippocampal levels of Brain-Derived Neurotrophic Factor (BDNF), Nerve Growth Factor (NGF), DNA oxidative damage, and the amount of Bax and Bcl2 apoptosis indices of rats exposed to AuNPs.
Methods: A total of 42 male Wistar rats were divided into four groups: control (30 days 0.5 mL saline), AuNPs (one time injection of 0.5 mL AuNPs, 200 ppm and 60 Nm + 30 days 0.5 mL saline), AuNPs+GING 50 (one time injection of 0.5 mL AuNPs, 200 ppm and 60 Nm + 30 days 0.5 mL density of gingerol 50 mg/kg), and AuNPs+GING100 (one time injection of 0.5 mL AuNPs, 200 ppm and 60 Nm + 30 days 0.5 mL density of gingerol 100 mg/kg). At the end of the treatment period, the hippocampal levels of NGF, BDNF, 8-hydroxy-desoxyguanosine (8-HOdG), and apoptotic indices of Bax and Bcl-2 were assessed with the ELISA method.
Results: Compared with the AuNPs group, hippocampal levels of BDNF, NGF, and Bcl-2 in rats in the AuNPs+GING 50 and AuNPs+GING 100 groups significantly increased dose-dependently. However, the hippocampal levels of Bax and 8-HOdG significantly decreased dose-dependently (P<0.05).
Conclusion: According to obtained results, gingerol may improve hippocampal BDNF and NGF levels in rats exposed to AuNPs, probably by reducing apoptosis and oxidative DNA damage.
http://bcn.iums.ac.ir/article-1-1629-en.pdf
Nanoparticles
Hippocampus gingerol
Brain derived neurotrophic factor
Nerve growth factor
eng
Iran University of Medical Sciences
Basic and Clinical Neuroscience
2008-126X
2228-7442
2021-05
12
3
309
314
article
Serum and Saliva Myelin Basic Protein as Multiple Sclerosis Biomarker
Mohammad Hossein Mirzaii-Dizgah
m.mashein@yahoo.com
1
Mohammad Reza Mirzaii-Dizgah
drmirzaiidizgah@gmail.com
2
Iraj Mirzaii-Dizgah
emirzaii@alumnus.tums.ac.ir
3
Student Research Committee, School of Dentistry, AJA University of Medical Sciences, Tehran, Iran.
Student Research Committee, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Department of Physiology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran.
Introduction: Multiple Sclerosis (MS) is presented with motor and sensory function loss. It is caused by demyelination and following axonal lesion. As Myelin Basic Protein (MBP) is one of the key elements of the myelin cover, we examined the level of MBP in serum, stimulated, and unstimulated saliva as a suitable biomarker for detecting MS.
Methods: A case-control study was performed in 29 healthy women and 32 definitive relapsing-remitting MS patients hospitalized in Imam Reza hospital, Tehran, Iran. MBP level was assayed in serum and stimulated and unstimulated whole saliva.
Results: MBP was expressed at a lower level in serum and stimulated saliva of MS patients compared to the control group. The serum MBP level had a considerable correlation with its stimulated saliva level. The receiver operating characteristic analysis showed significant diagnostic ability for MBP to discriminate MS patients with serum and stimulated saliva from controls.
Conclusion: Serum and saliva level of MBP is lower in MS, so it may be considered a potential biomarker in MS.
http://bcn.iums.ac.ir/article-1-1573-en.pdf
Multiple sclerosis
Myelin Basic Protein
Saliva
Serum
eng
Iran University of Medical Sciences
Basic and Clinical Neuroscience
2008-126X
2228-7442
2021-05
12
3
315
324
article
Differentiation of Mesenchymal Stem Cells Derived From Human Adipose Tissue Into Cholinergic-like Cells: An in Vitro Study
Davood Sanooghi
1
Naser Amini
2
Fereshteh Azedi
fazeditehrani@gmail.com
3
Zohreh Bagher
4
Asghar Parvishan
5
Abolfazl Lotfi
6
Nooshin Rashidi
7
Erfan Lotfi
8
Forough Azam Sayahpour
9
Faezeh Faghihi
faghihi.f@iums.ac.ir
10
Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
ENT and Head & Neck Research Center, The Five Basic Sensory Institute, Hazrat Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran.
Department of Neuroscience, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
Damavand Agricultural College, Technical and Vocational University, Tehran, Iran.
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
School of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, Academic Center for Education, Culture and Research, Tehran, Iran.
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
Introduction: Cholinergic-associated diseases currently constitute a significant cause of neurological and neurodegenerative disabilities. As the drugs are not efficient in improving the suffered tissues, stem cell treatment is considered an effective strategy for substituting the lost cells.
Methods: In the current study, we set out to investigate the differentiation properties of human Adipose-Derived Mesenchymal Stem Cells (AD-MSCs) into cholinergic-like cells by two morphogens of Retinoic Acid (RA) and Sonic Hedgehog (Shh) using a three-step in vitro procedure. The results were evaluated using real-time PCR, flow cytometry, and immunocytochemistry for two weeks.
Results: Our data showed that the cells could express cholinergic specific markers, including Islet-1, Acetylcholinesterase (AChE), SMI-32, and Nestin, at mRNA and protein levels. We could also quantitatively evaluate the expression of Islet-1, AChE, and Nestin at 14 days post-induction using flow cytometry.
Conclusion: Human AD-MSCs are potent cells to differentiate into cholinergic-like cells in the presence of RA and Shh through a three-step protocol. Thus, they could be a suitable cell candidate for the regeneration of cholinergic-associated diseases. However, more functional and electrophysiological analyses are needed in this regard.
http://bcn.iums.ac.ir/article-1-1589-en.pdf
Cholinergic differentiation
Adipose-derived mesenchymal stem cells
Retinoic acid
Sonic hedgehog
eng
Iran University of Medical Sciences
Basic and Clinical Neuroscience
2008-126X
2228-7442
2021-05
12
3
325
338
article
Pharmacological and Molecular Evidence of Neuroprotective Curcumin Effects Against Biochemical and Behavioral Sequels Caused by Methamphetamine: Possible Function of CREB-BDNF Signaling Pathway
Mina Gholami
1
Farzad Hozuri
2
Setayesh Abdolkarimi
3
Mahsa Mahmoudi
4
Majid Motaghinejad
dr.motaghinejad6@gmail.com
5
Sepideh Safari
6
Samira Sadr
7
Department of Medicinal Chemistry, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Chemistry, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.
Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Chemistry, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.
Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Chemistry, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.
Research Center for Addiction and Risky Behaviors (ReCARB), Iran Psychiatric Center, Iran University of Medical Sciences, Tehran, Iran.
Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran.
Department of Research and Development, Parsian-Exir-Aria Pharmaceutical Company, Tehran, Iran.
Introduction: The neuroprotective impact of curcumin and the role of CREB (Cyclic AMP Response Element Binding protein)-BDNF (Brain-Derived Neurotrophic Factor) signaling pathway was evaluated in Methamphetamine (METH)-induced neurodegeneration in rats.
Methods: Sixty adult male rats were randomly divided into 6 groups. While normal saline and 10 mg/kg METH were administered intraperitoneally in groups 1 and 2, groups 3, 4, 5, and 6 received METH (10 mg/kg) and curcumin (10, 20, 40, and 80 mg/kg, respectively) simultaneously. Morris water maze test was administered, and oxidative hippocampal, antioxidant, inflammatory, apoptotic, and CREB and BDNF were assessed.
Results: We found that METH disturbs learning and memory. Concurrent curcumin therapy (40 and 80 mg/kg) decreased cognitive disturbance caused by METH. Multiple parameters, such as lipid peroxidation, the oxidized form of glutathione, interleukin 1 beta, tumor necrosis factor-alpha, and Bax were increased by METH therapy, while the reduced type of glutathione, Bcl-2, P-CREB, and BDNF concentrations in the hippocampus were decreased.
Conclusion: Different doses of curcumin adversely attenuated METH-induced apoptosis, oxidative stress, and inflammation but enhanced the concentrations of P-CREB and BDNF. The neuroprotection caused by curcumin against METH-induced neurodegeneration is mediated through P-CREB-BDNF signaling pathway activation.
http://bcn.iums.ac.ir/article-1-1382-en.pdf
Methamphetamine
Curcumin
Neurodegeneration
Cyclic AMP response element binding protein
Brain-derived neurotrophic factor
eng
Iran University of Medical Sciences
Basic and Clinical Neuroscience
2008-126X
2228-7442
2021-05
12
3
339
348
article
High-dose Vitamin D Supplementation and Improvement in Cognitive Abilities, Insomnia, and Daytime Sleepiness in Adolescent Girls
Afsane Bahrami
afsbahrami91@yahoo.com
1
Fariborz Rezaeitalab
2
Seyed Kazem Farahmand
3
Zahra Mazloum Khorasani
4
Seyed Mostafa Arabi
5
Hamidreza Bahrami-Taghanaki
6
Gordon A. Ferns
7
Majid Ghayour-Mobarhan
8
Cellular and Molecular Research Center, School of Medicine, Birjand University of Medical Sciences, Bijand, Iran.
Department of Neurology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Traditional Medicine, School of Persian and Complementary Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Endocrine Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Biochemistry of Nutrition Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Chinese and Complementary Medicine Research Center, School of Traditional and Complementary Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Division of Medical Education, School of Brighton & Sussex Medical, University of Brighton and the University of Sussex, Falmer, Brighton, Sussex, UK.
Metabolic Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Introduction: Vitamin D may affect the modulation of signaling pathways in the central nervous system. We aimed to evaluate the effect of high-dose vitamin D supplementation on neuropsychological functions in female adolescents.
Methods: We studied the effects of 9 weeks of vitamin D supplementation (50000 IU vitamin D3 [cholecalciferol]/week) on cognitive abilities and sleep disorders in 940 adolescent girls.
Results: Oral vitamin D supplementation improved cognitive abilities, including memory, inhibitory control, selective attention, decision making, planning, sustained attention, and cognitive flexibility in healthy adolescent girls (P<0.001). The prevalence of subjects with insomnia after intervention fell from 15.0% to 11.3%. Similar results were also found for the prevalence of sleepiness (15.6% reduced to 14.7%), or cases with both insomnia and sleepiness (8.0% reduced to 6.1%; P<0.05).
Conclusion: High dose of vitamin D can improve cognitive abilities and alleviate insomnia and daytime sleepiness in adolescent girls. Further investigations are required on different population groups (age and gender) to determine the sustainability of these effects. The value of vitamin D therapy in other neurological disorders would also be of research interest.
http://bcn.iums.ac.ir/article-1-1538-en.pdf
Vitamin D
Cognition
Adolescents
Sleepiness
Insomnia
eng
Iran University of Medical Sciences
Basic and Clinical Neuroscience
2008-126X
2228-7442
2021-05
12
3
349
360
article
Effects of Eight Weeks of Resistance Exercises on Neurotrophins and Trk Receptors in Alzheimer Model Male Wistar Rats
Gholamhasan Jafarzadeh
jafarzadehasan@yahoo.com
1
Saeid Shakerian
sashakeryan@gmail.com
2
Yaghoob Farbood
farbood_y@yahoo.com
3
Mohsen Ghanbarzadeh
ghanbarzadeh213@gmail.com
4
Department of Sport Physiology, Faculty of Sports Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Department of Sport Physiology, Faculty of Sports Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Department of Physiology, Khalij Fars Physiology Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Sport Physiology, Faculty of Sports Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Introduction: This study evaluates the effects of 8 weeks of resistance exercises on the expression of neurotrophins and Trk receptors in Alzheimer model male Wistar rats.
Methods: For this purpose, 32 mature male Wistar rats with a mean weight of 230-280 g were chosen and divided into Alzheimer and Sham groups. The rats in the sham group received normal saline, while the ones in the Alzheimer group received streptomycin via intraventricular injection. These rats were then divided into the following four subgroups: 1) resting sham, 2) exercising sham, 3) resting Alzheimer, and 4) exercising Alzheimer. The two exercising rat subgroups exercised three times a week for 8 weeks. A weight was attached to their tails, and they had to carry it on a 26-step ladder in each cycle. Resting groups were handled every day to minimize the effects of stress. At the end of the eighth week and 24 hours after the last exercise session (to avoid the effects of the last exercise session), the rats were put under deep anesthesia and beheaded. Hippocampus tissues were precisely extracted, and samples were sent to the laboratory for molecular and cellular tests. To investigate gene expression, quantitative RT-PCR was used.
Results: The tests for comparing the mean values of BDNF, NT3, NGF, TrkA, and TrkB in two rat groups showed that with error levels of less than 5%, there is a significant difference in the amounts of BDNF, NT3, NGF, TrkA, and TrkB between exercising rats and resting ones. These amounts were much higher in the exercising Alzheimer rats group.
Conclusion: Eight weeks of resistance exercises increased the expression of BDNF, NT3, and NGF genes and TrkA and TrkB receptors in Alzheimer model Wistar rats.
http://bcn.iums.ac.ir/article-1-1606-en.pdf
Resistance exercises
Neurotrophins
Trk receptors
Alzheimer
Rat
eng
Iran University of Medical Sciences
Basic and Clinical Neuroscience
2008-126X
2228-7442
2021-05
12
3
361
372
article
Comparing the Effects of Rosemary Extract and Treadmill Exercise on the Hippocampal Function and Antioxidant Capacity in Old Rats
Homa Rasoolijazi
hjaz.2010@yahoo.com
1
Sattar Norouzi Ofogh
zenon1366@gmail.com
2
Shima Ababzadeh
shimaababzadeh@gmail.com
3
Mehdi Mehdizadeh
Mehdizadeh.m@iums.ac.ir
4
Fatemeh Shabkhiz
shabkhiz@ut.ac.ir
5
Cellular & Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
Department of Neuroscience, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
Cellular & Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
Cellular & Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
Department of Neuroscience, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
Introduction: A sequence of time-dependent changes can affect the brain’s functional capacity.
This study aimed at investigating the effects of Forced Aerobic Exercise (FAE) versus the Rosemary Extract (RE) on the learning abilities and oxidative stress modulation in rats.
Methods: Young and old rats received daily FAE and RE for 3 months. Using the Passive Avoidance (PA) test, we evaluated the learning and memory of the rats by Step-Through Latency (STL) score. We measured the Superoxide Dismutase (SOD), Glutathione Peroxidase (GPx), Catalase (CATA), Malondialdehyde (MDA) enzymes levels, and Total Antioxidant Capacity (TAC) in the hippocampus
Results: FAE could significantly increase the STL score (P<0.001) among old rats similar to the rosemary extract consumption. The SOD, GPx, and CATA enzyme activities and the level of TAC significantly increased by the treatments (exercise: P<0.001 for SOD and TAC and P<0.05 for CATA, exercise/rosemary: P<0.001 for all enzymes, and rosemary: P<0.01 for SOD and TAC). Furthermore, the MDA level significantly decreased by the treatments (exercise and exercise/rosemary: P<0.001, rosemary: P<0.01). The partial Pearson test revealed the significant positive correlations between the score of STL (day 2) with the SOD (P<0.01) and TAC (P<0.05) levels and negative correlations between the MDA level and STL score in both days (P<0.05 for the first day and P<0.001 for the second day).
Conclusion: Similar to the rosemary extract, FAE could increase the working memory and antioxidants activity in old rats in 3 months.
http://bcn.iums.ac.ir/article-1-1638-en.pdf
Cognition
Antiox idant
Enzymes
Aerobic exercise
Aging
eng
Iran University of Medical Sciences
Basic and Clinical Neuroscience
2008-126X
2228-7442
2021-05
12
3
373
382
article
The Effect of Resistance Training and Berberine Chloride on the Apoptosis-related Unfolded Protein Response Signaling Pathway in the Hippocampus of Diazinon-poisoned Rats
Ali Esfandiarifar
ali.esfandiyari.spr@iauctb.ac.ir
1
Mohammad Ali Azarbayjani
m_azarbayjani@iauctb.ac.ir
2
Maghsood Peeri
m.peeri@iauctb.ac.ir
3
Seyed Behnamedin Jameie
Jameie.sb@iums.ac.ir
4
Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Neuroscience Research Center, Iran University of Medical Sciences, Tehran, Iran.
Introduction: Diazinon is one of the most widely-used organophosphate pesticides in the world. This toxin enters the body in various ways and induces oxidative stress in various tissues. It has been proved that activation of Unfolded Protein Response (UPR) under oxidative stress is a steady mechanism for maintaining cell function and survival. Therefore, the present study aimed to review the effect of Resistance Training (RT) and Berberine Chloride (BC) on the apoptosis-related UPR signaling pathway in the hippocampus of diazinon-poisoned rats.
Methods: In this experimental study, 40 male Wistar rats weighing 250 ±50 g were randomly divided into eight groups of five rats of 1) diazinon + 2 mg/kg BC + RT, 2) diazinon + 15 mg/kg BC + RT, 3) diazinon, 4) diazinon + RT, 5) diazinon + 2 mg/kg BC, 6) diazinon + 15 mg/ kg BC, 7) healthy control, and 8) sham. The groups were treated for 5 weeks. At the end of the fifth week, ATF-4, ATF-6, and CHOP gene expression in hippocampus tissue were measured by quantitative real-time RT-PCR.
Results: Diazinon significantly increased the expression of ATF-4, ATF-6, and CHOP in the hippocampus tissue of rats. Administrating 15 mg/kg BC with RT significantly decreased these genes, indicating a decrease in the rate of apoptosis in the hippocampus.
Conclusion: This study showed that RT and BC have a protective effect against diazinon-induced toxicity in the hippocampus.
http://bcn.iums.ac.ir/article-1-1665-en.pdf
Berberine chloride
Diazinon
Resistance training
Activating Transcription Factor 4 (ATF-4)
Activating Transcription Factor 6 (ATF-6)
CHOP
eng
Iran University of Medical Sciences
Basic and Clinical Neuroscience
2008-126X
2228-7442
2021-05
12
3
383
394
article
β-Amyloid Formation, Memory, and Learning Decline Following Long-term Ovariectomy and Its Inhibition by Systemic Administration of Apigenin and β-Estradiol
Seyed Behnamedin Jameie
jameie.sb@iums.ac.ir
1
Abbas Pirasteh
apirasteh6@gmail.com
2
Ali Naseri
Ali.N5@gmail.com
3
Melika Sadat Jameie
behjame@gmail.com
4
Mona Farhadi
monafarhadi@yahoo.com
5
Javad Fahanik Babaee
Fahanikbabaee1358@gmail.com
6
Leila Elyasi
Elyasy_leila@yahoo.com
7
Neuroscience Research Center, Iran University of Medical Sciences, Tehran, Iran.
Department of Psychology, Faculty of Humanities, Firoozabad Branch, Islamic Azad University, Fars, Iran.
Department of Psychology, Faculty of Humanities, Firoozabad Branch, Islamic Azad University, Fars, Iran.
Department of Anatomy, Faculty of Medicine, Shahid Behshti University of Medical Sciences, Tehran, Iran.
Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran.
Department of Physiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Department of Anatomy, Neuroscience Research Center, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.
Introduction: The increasing cases of Alzheimer Disease (AD) has caused numerous problems. The risk of developing AD increases in menopausal women, too. Apigenin and β-estradiol are effective antioxidant and neuroprotective agents. We conducted the present study to explore their combined effects on β-amyloid plaque formation, memory, and learning in ovariectomized rats.
Methods: Forty-two Wistar rats were randomly assigned into 6 groups: 1) ovariectomized (OVX), 2) OVX + apigenin, 3) OVX + β-estradiol, 4) OVX + apigenin + β-estradiol, 5 &6) vehicle shams for E2 and API , and 7) surgical sham. Treatment was done with apigenin and β-estradiol. Then, we studied the formation of β-amyloid plaques, neuronal density in the hippocampus area, apoptosis, memory, and learning.
Results: Findings showed the significant formation of β-amyloid plaques in the hippocampus of OVX animals and their memory impairment. Apigenin and β-estradiol significantly reduced the number of β-amyloid plaques, as well as the symptoms of memory impairment and learning, and decreased the expression of caspase-3 in treated animals.
Conclusion: Accordingly, β-estradiol and apigenin could have more potent therapeutic effects on AD.
http://bcn.iums.ac.ir/article-1-1820-en.pdf
β-Amyloid plaques
β-Estradiol
Apigenin
Ovariectomy
eng
Iran University of Medical Sciences
Basic and Clinical Neuroscience
2008-126X
2228-7442
2021-05
12
3
395
408
article
Glycine/NMDA Receptor Pathway Mediates the Rapid-onset Antidepressant Effect of Alkaloids From Trichilia Monadelpha
Kennedy Kwami Edem Kukuia
edemkennedy@yahoo.com
1
Jeffrey Amoako Mensah
jeffreyamoako.mensah@utah.edu
2
Patrick Amoateng
pamoateng@ug.edu.gh
3
Dorcas Osei-Safo
doseisafo@ug.edu.gh
4
Awo Efua Koomson
diamondefua@ymail.com
5
Joseph Torbi
jtorbi@st.ug.edu.gh
6
Donatus Wewura Adongo
donatusadongo@yahoo.com
7
Elvis Ofori Ameyaw
8
Inemesit Okon Ben
9
Seth Kwabena Amponsah
10
Kwasi Agyei Bugyei
11
Isaac Julius Asiedu-Gyekye
12
Department of Medical Pharmacology, University of Ghana Medical School, College of Health Sciences, University of Ghana, Korle Bu-Accra, Ghana.
Department of Pharmacology and Toxicology, College of Pharmacy, University of Utah, Salt Lake City, UT, U.S. A.
Department of Pharmacology and Toxicology, School of Pharmacy, College of Health Sciences, University of Ghana, Legon-Accra, Ghana.
Department of Chemistry, School of Physical and Mathematical Sciences, College of Basic and Applied Sciences, University of Ghana, Legon-Accra, Ghana.
Department of Pharmacology and Toxicology, School of Pharmacy, College of Health Sciences, University of Ghana, Legon-Accra, Ghana.
Department of Pharmacology and Toxicology, School of Pharmacy, College of Health Sciences, University of Ghana, Legon-Accra, Ghana.
Department of Pharmacology and Toxicology, School of Pharmacy, University of Health and Allied Sciences, Ho, Ghana.
Department of Pharmacology, School of Pharmacy and Pharmaceutical Sciences, University of Cape Coast, Cape Coast, Ghana.
Department of Pharmacology and Toxicology, School of Pharmacy, University of Health and Allied Sciences, Ho, Ghana.
Department of Medical Pharmacology, University of Ghana Medical School, College of Health Sciences, University of Ghana, Korle Bu-Accra, Ghana.
Department of Medical Pharmacology, University of Ghana Medical School, College of Health Sciences, University of Ghana, Korle Bu-Accra, Ghana.
Department of Pharmacology and Toxicology, School of Pharmacy, College of Health Sciences, University of Ghana, Legon-Accra, Ghana.
Introduction: Major depressive disorder is often associated with suicidal tendencies, and this condition accentuates the need for rapid-acting antidepressants. We previously reported that Alkaloids (ALK) from Trichilia monadelpha possess antidepressant action in acute animal models of depression and that this effect is mediated through the monoamine and L-arginine-NO-cGMP pathways. This study investigated the possible rapid-onset antidepressant effect of ALK from T. monadelpha and its connection with the glycine/NMDA receptor pathway.
Methods: The onset of ALK action from T. monadelpha was evaluated using the Open Space Swim Test (OSST), a chronic model of depression. The modified forced swimming and tail suspension tests were used to assess the effect of the ALK on the glycine/NMDA receptor pathway. The Instutute of Cancer Research (ICR) mice were treated with either ALK (30–300 mg/kg, orally [PO]), imipramine (3–30 mg/kg, PO), fluoxetine (3–30 mg/kg, PO), or saline. To identify the role of glycine/NMDA receptor pathway in the effect of ALK, we pretreated mice with a partial agonist of the glycine/NMDA receptor, D-cycloserine (2.5 mg/kg, intraperitoneally [IP]), and an agonist of glycine/NMDA receptor, D-serine (600 mg/kg, IP), before ALK administration.
Results: ALK reversed immobility in mice after the second day of drug treatment in the OSST. In contrast, there was a delay in the effects induced by fluoxetine and imipramine. ALK also increased mean swimming and climbing scores in mice. ALK was more efficacious than imipramine and fluoxetine in reducing immobility and increasing distance traveled. It is noteworthy that ALK was less potent than fluoxetine and imipramine. D-cycloserine potentiated mobility observed in the ALK- and fluoxetine-treated mice. In contrast, D-serine decreased mobility in the ALK-treated mice.
Conclusion: The study results suggest that ALK from T. monadelpha exhibits rapid antidepressant action in mice, and the glycine/NMDA receptor pathway possibly mediates the observed effect.
http://bcn.iums.ac.ir/article-1-1907-en.pdf
Glycine/NMDA receptor
Open space swim test
Rapid-acting antidepressant
Trichilia
Alkaloids
eng
Iran University of Medical Sciences
Basic and Clinical Neuroscience
2008-126X
2228-7442
2021-05
12
3
409
420
article
Analgesic Effect of Bumetanide on Neuropathic Pain in Patients With Spinal Cord Injury
Farinaz Nasirinezhad
fnasirinezhad12@yahoo.com
1
Leila Zarepour
leilazarepour1@yahoo.com
2
Mahmoudreza Hadjighassem
hajighasem1398@yahoo.com
3
Zeinab Gharaylou
gharaylouz12@yahoo.com
4
Hossin Majedi
majedih12@yahoo.com
5
Fatemeh Ramezani
framezani2014@yahoo.com
6
Department of Physiology, Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran.
Department of Physiology, Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran.
Brain and Spinal cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran.
Brain and Spinal cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran.
Brain and Spinal cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran.
Department of Physiology, Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran.
Introduction: The current study evaluated the analgesic effects of bumetanide as an adjunctive in the management of neuropathic pain following Spinal Cord Injury (SCI). The peripheral expression of Na-K-Cl Cotransporter-1 (NKCC1) and K-Cl Cotransporter-2 (KCC2) genes in polymorphonuclear lymphocytes (PMLs) was assessed as a possible biomarker indicating central mechanisms underlying the observed response.
Methods: Through an open-label, single-arm, pilot trial of bumetanide (2 mg/d), an add-on treatment was conducted on 14 SCI patients for 19 weeks. This study consisted of 3 phases: pre-treatment (1 month), titration (3 weeks), and active treatment (4 months). Ultimately, 9 patients completed the study. The primary outcome variables were the endpoint pain score using the Numeric Rating Scale (NRS), and also the short-form of the McGill pain questionnaire. Secondary endpoints included the short-form of the health survey that assesses the quality of life. Blood samples were collected and used for determining the expression of NKCC1 and KCC2 genes in transcription and translation levels.
Results: Bumetanide treatment significantly decreased average pain intensity according to the NRS and the short-form of the McGill pain questionnaire scores. Baseline expression of KCC2 protein was low between groups and increased significantly following treatment (P<0.05). In the current study, pain improvement was accompanied by the greater mean change from the baseline (improvement) for the overall quality of life.
Conclusion: These data highlighted the analgesic effect of bumetanide on neuropathic pain and indicated the potential role of the upregulation of KCC2 protein and involvement of GABAergic disinhibition in producing neuropathic pain.
http://bcn.iums.ac.ir/article-1-1607-en.pdf
Bumetanide
Na-K-Cl Cotransporter-1 (NKCC1)
K-Cl Cotransporter-2 (KCC2)
Neuropathic pain
Spinal cord injury
GammaAminobutyric Acid (GABA)
eng
Iran University of Medical Sciences
Basic and Clinical Neuroscience
2008-126X
2228-7442
2021-05
12
3
421
426
article
Report of a Novel Bilateral Variation of Sciatic and Inferior Gluteal Nerve: A Case Study
Rahim Golmohammadi
rahimgolmohammadi@yahoo.com
1
Ali Delbari
alidel.del@gmail.com
2
Department of Anatomical Sciences, Cellular and Molecular Research Center, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran.
Department of Anatomical Sciences, Cellular and Molecular Research Center, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran.
Introduction: The sciatic nerve is the thickest nerve of the sacral plexus which innervates many muscles and vast areas of the skin of the lower limb. It leaves the pelvis via the greater sciatic foramen, emerges into the gluteal region by passing under the piriformis muscle, and descends beneath the gluteus maximus to divide into its terminal branches; the tibial and common peroneal nerve at the superior angle of the popliteal fossa. In some cases, the sciatic nerve divides into the tibial and common peroneal nerves at a higher level and one of them or both passes through or over the piriformis muscle.
Case Presentation: We find an interesting bilateral variation of sciatic nerve accompanying a very thick inferior gluteal nerve on the right side and unusual route and branching of tibial and common peroneal nerves on the left side.
Conclusion: As in conditions like intramuscular injections, gluteal surgeries, and piriformis syndrome such variations may increase the risk of injury, it is important for the medical team to be aware of them. In this paper, by reporting many variations in a cadaver, we emphasize the importance of anatomical variations, especially for surgeons and nurses.
http://bcn.iums.ac.ir/article-1-1530-en.pdf
Sciatic nerve
Inferior gluteal nerve
Anatomic variation