Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1397
8
1
gregorian
2018
11
1
9
6
online
1
fulltext
en
Effects of Jobelyn® on Isoniazid-Induced Seizures, Biomarkers of Oxidative Stress and Glutamate Decarboxylase Activity in Mice
Behavioral Neuroscience
Behavioral Neuroscience
Original
Original
<div style="text-align: justify;"><p></p><strong>Introduction:</strong> Isoniazid-induced seizure, often described as Status Epilepticus (SE), is an emergency condition characterized by repeated convulsive episodes that responds poorly to the currently available anticonvulsant drugs. The current study aimed at ascertaining the effect of Jobelyn® (JB), an African dietary supplement, on seizures, altered oxidative stress, and glutamate decarboxylase activity induced by isoniazid in mice.<br>
<strong>Methods:</strong> A total of 6 mice received JB (10-50 mg/kg, PO), pyridoxine (300 mg/kg), diazepam (5 mg/kg), or distilled water (10 mL/kg) 30 minutes prior to the induction of SE with injection of isoniazid (300 mg/kg, IP). Thereafter, the mice were observed for the onset of convulsions for a period of two hours. Moreover, the effect of JB on Glutamate Decarboxylase (GAD) activity and biomarkers of oxidative stress (glutathione and malondialdehyde) was also evaluated in the brain homogenates of another set of isoniazid-treated mice.<br>
<strong>Results:</strong> JB (50 mg/kg, PO) prolonged the latency to convulsions, but could not prevent the occurrence of seizure episodes caused by isoniazid. Moreover, JB neither showed any protection against death nor delayed the latency to death caused by isoniazid. However, this dose of JB positively modulated the concentrations of malondialdehyde and glutathione in the brains of mice treated with isoniazid. The activity of GAD, the enzyme responsible for GABA synthesis, increased by JB, which suggested enhanced GABAergic neurotransmission.<br>
<strong>Conclusion:</strong> The current study findings suggest that JB prolongs the latency to convulsions, enhances GABAergic neurotransmission, and demonstrates anti-oxidative effect in isoniazid-treated mice.</div>
Sorghum bicolor, Seizures, Isoniazid, Glutamate decarboxylase, GABA
389
396
http://bcn.iums.ac.ir/browse.php?a_code=A-10-334-5&slc_lang=en&sid=1
Stephen
Asehinde
hapler4real2001@gmail.com
13700319475328460018399
13700319475328460018399
No
Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Abayomi
Ajayi
yomexj@yahoo.com
13700319475328460018400
13700319475328460018400
No
Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Adewale
Bakre
jasbe2000@yahoo.com
13700319475328460018401
13700319475328460018401
No
Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Osarume
Omorogbe
omorogbeosarume@yahoo.com
13700319475328460018402
13700319475328460018402
No
Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Adaeze
Adebesin
13700319475328460018403
13700319475328460018403
No
Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Solomon
Umukoro
umusolo@yahoo.com
13700319475328460018404
13700319475328460018404
Yes
Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Ibadan, Nigeria.