Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1396
4
1
gregorian
2017
7
1
8
4
online
1
fulltext
en
Serum Proteomic Profiling of Obsessive-Compulsive Disorder, Washing Subtype: A Preliminary Study
Behavioral Neuroscience
Behavioral Neuroscience
Original
Original
<p style="text-align: justify;"><strong>Introduction:</strong> Obsessive-Compulsive Disorder (OCD) is a disabling mental condition that its proteomic profiling is not yet investigated. Proteomics is a valuable tool to discover biomarker approaches. It can be helpful to detect protein expression changes in complex disorders such as OCD.<br>
<strong>Methods:</strong> Here, by the application of 2D gel electrophoresis (2DE), a pilot study of serum proteome profile of females with washing subtype of OCD was performed. Serum samples were obtained from females with washing subtype of OCD. Following the protein extraction from the serum with acetone perception, the samples were subjected to 2DE for separation based on pI and molecular weight (MW) with triple replications. Finally, the protein spots were visualized using Coomassie blue staining method and analyzed by Progenesis SameSpots software. Furthermore, protein-protein interaction (PPI) network analysis was handled by the application of Cytoscape software. <br>
<strong>Results:</strong> The results suggested that 41 matched spots demonstrated significant expression alterations among which 5 proteins including immunoglobulin heavy constant alpha-1 (IGHA1), apolipoprotein A-4 (APOA4), haptoglobin (HP), protein α-1-antitrypsin (SERPINA1), and component 3 (C3) were identified by database query. Additionally, PPI network analysis indicated the central role of SERPINA1 and C3 in the network integrity. However, albumin (ALB), amyloid precursor protein (APP), and protein α-1-antitrypsin (APOA1) proteins were important in OCD PPI network as well. The identified proteins were related to 3 processes: acute-phase response, hydrogen peroxide catabolic process, and regulation of triglyceride metabolic process.<br>
<strong>Conclusion:</strong> It was concluded that these proteins may have a fundamental role in OCD pathogenesis. Moreover, the dysregulation of inflammatory and antioxidant systems in OCD risk was suggested by the current study. However, evaluation of bigger sample sizes and application of mass spectrometry are essential requirements to confirm this preliminary evaluation.</p>
Obsessive-compulsive disorder, Washing subtype, Serum, Proteomics, Protein-Protein interaction network analysis
307
316
http://bcn.iums.ac.ir/browse.php?a_code=A-10-941-1&slc_lang=en&sid=1
Mona
Zamanian-Azodi
mona.azodi@gmail.com
13700319475328460010892
13700319475328460010892
No
Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mostafa
Rezaei-Tavirani
tavirany@yahoo.com
13700319475328460010893
13700319475328460010893
Yes
Proteomics Research Center, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Naser
Nejadi
nejadi.naser@gmail.com
13700319475328460010894
13700319475328460010894
No
Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Afsaneh
Arefi Oskouie
a_arefioskouie@yahoo.com
13700319475328460010895
13700319475328460010895
No
Proteomics Research Center, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Faird
Zayeri
fzayeri@yahoo.com
13700319475328460010896
13700319475328460010896
No
Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mostafa
Hamdieh
mostafa_hamdieh@yahoo.com
13700319475328460010897
13700319475328460010897
No
Taleghani Hospital, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Akram
Safaei
akramsafaei.134@gmail.com
13700319475328460010898
13700319475328460010898
No
Proteomics Research Center, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Majid
Rezaei-Tavirani
tavirani_m@yahoo.com
13700319475328460010899
13700319475328460010899
No
Department of Surgery, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Alireza
Ahmadzadeh
rezaei.tavirani@ibb.ut.ac.ir
13700319475328460010900
13700319475328460010900
No
Proteomics Research Center, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Alireza
Amouzandeh-Nobaveh
mona_az_2001@yahoo.com
13700319475328460010901
13700319475328460010901
No
Department of Microbiology, School of Medicine, Arak University of Medical Sciences, Arak, Iran.
Farshad
Okhovatian
farshadokhovatian1965@gmail.com
13700319475328460010902
13700319475328460010902
No
Physiotherapy Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.