Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1397
8
1
gregorian
2018
11
1
9
6
online
1
fulltext
en
Different Expressions of Specific Transcription Factors of Th1 (T-bet) and Th2 cells (GATA-3) by Peripheral Blood Mononuclear Cells From Patients With Multiple Sclerosis
Cellular and molecular Neuroscience
Cellular and molecular Neuroscience
Original
Original
<p style="text-align: justify;"><strong>Introduction:</strong> Multiple Sclerosis (MS) is an inflammatory disorder caused by self-reactive Th1 lymphocytes, while Th2 cells may confer protection. The Th1 and Th2 cell differentiation are regulated by specific transcription factors, especially T-bet and GATA-3, respectively. This investigation aimed to measure the T-bet and GATA-3 expression by Peripheral Blood Mononuclear Cells (PBMCs) obtained from MS patients after specific and non-specific in vitro stimulation.<br>
<strong>Methods: </strong>The PBMCs were separated from 22 patients with MS and 20 healthy individuals. They were cultured at 37°C for 24 h in the absence of a stimulator or in the presence of Myelin oligodendrocyte Glycoprotein (MOG) or Phytohemagglutinin (PHA) at a concentration of 10 μg/mL. Then the T-bet and GATA-3 expression was measured by real time-PCR.<br>
<strong>Results:</strong> The T-bet expression was enhanced, while the GATA-3 expression diminished. Therefore the expression of T-bet/GATA-3 ratio diminished in not-stimulated, MOG-stimulated and PHA-stimulated PBMCs from MS patients compared with equal cultures from the healthy individuals (P<0.01, P<0.01 and P<0.01, for T-bet; P<0.03, P<0.01 and P<0.02, for GATA-3; P<0.01, P<0.001 and P<0.01 for T-bet/GATA-3 ratio, respectively). The not-stimulated, MOG-stimulated, and PHA-stimulated PBMCs from men with MS expressed higher amounts of GATA-3 than equal cells from MS women (P<0.05, P<0.05 and P<0.01, respectively).<br>
<strong>Conclusion:</strong> These results probably indicate an imbalance in Th1/Th2 cells in the level of transcription factors with a tendency toward Th1 cells in MS. The clinical utilization of the transcription factors as novel biomarkers of MS should be evaluated in further studies.</p>
<p></p>
Multiple Sclerosis, Th1, Th2, T-bet, GATA-3
458
469
http://bcn.iums.ac.ir/browse.php?a_code=A-10-900-1&slc_lang=en&sid=1
Zahra
Etesam
zahraeatesam@gmail.com
13700319475328460018586
13700319475328460018586
No
Neurology Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Maryam
Nemati
nemati_m22@yahoo.com
13700319475328460018587
13700319475328460018587
No
Department of Immunology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Mohammad-Amin
Ebrahimizadeh
Amin.68.2@gmail.com
13700319475328460018588
13700319475328460018588
No
Department of Immunology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Hossain-Ali
Ebrahimi
hebrahimi@kmu.ac.ir
13700319475328460018589
13700319475328460018589
No
Neurology Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Hossain
Hajghani
h_hajghani@yahoo.com
13700319475328460018590
13700319475328460018590
No
Department of Immunology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Tahereh
Khalili
taherehkha2131@yahoo.com
13700319475328460018591
13700319475328460018591
No
Department of Biochemistry, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Abdollah
Jafarzadeh
Jafarzadeh14@yahool.com
13700319475328460018592
13700319475328460018592
Yes
Department of Immunology, School of Medical, Rafsanjan University of Medical Sciences, Rafsanjan, Iran