Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1394
1
1
gregorian
2015
4
1
6
2
online
1
fulltext
en
Assessment of the Optimal Stimulus Pattern to Achieve Rapid Dorsal Hippocampal Kindling in Rats
Behavioral Neuroscience
Behavioral Neuroscience
Original
Original
<b>
Introduction:</b> Although hippocampus is the most famous brain area involved in temporal lobe
epilepsy, hippocampal kindling (HK) develops very slowly. Hence, rapid kindling is usually
preferred to the traditional kindling and it is widely used. In this article we aimed at finding the
optimal stimulus pattern, which yields the fastest HK rate. <div><b>Methods:</b> Stimulus patterns with different duration (2, 3, 5 and 10 s) and inter-train interval (ITI)
(5, 10 and 30 min) as well as number of trains in 24 h (8 and 12) were exerted to rats’ dorsal
hippocampus. The stimuli were continued until appearance of 3 consecutive generalized seizures
or maximum 7 days stimulations. </div><div><b>Results:</b> While the protocol with train duration of 10 s and ITI of 30 min caused the fastest
kindling rate and the most growth of afterdischarges, the protocol with train duration of 5 s and
ITI of 5 min was the most time-consuming protocol among protocols tested. </div><div><b>Discussion: </b>Rapid HK develops with a time course of days compared to weeks in traditional
kindling. Train duration and inter-train interval are key factors for rapid HK. Among the patterns,
12 trains/24h of 50Hz monophasic square wave with 10 s duration and 30 min interval between
trains, is the best stimulus pattern for eliciting rapid dorsal HK.
</div>
Dorsal hippocampus, Electrical kindling, Stimulation protocol
107
112
http://bcn.iums.ac.ir/browse.php?a_code=A-10-662-2&slc_lang=en&sid=1
Fataemeh
Etemadi
negin112837@yahoo.com
1370031947532846005294
1370031947532846005294
No
Department of Physiology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran
Mohammad
Sayyah
sayyahm2@pasteur.ac.ir
1370031947532846005295
1370031947532846005295
Yes
Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran
Hamid
Gholami Pourbadi
gholamipour@gmail.com
1370031947532846005296
1370031947532846005296
No
Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran
Vahab
Babapour
Bp.Vahab@gmail.com
1370031947532846005297
1370031947532846005297
No
Department of Physiology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran