en Behavioral Neuroscience Behavioral Neuroscience Original Original <b> Introduction:</b> Borago officinalis flower (borage) is a known sedative in herbal medicine the aim of the present study was to evaluate the antinociceptive effect of borage hydroalcoholic extract in formalin test male rats. <div><b>Methods:</b> Fifty-six adult male albino Wistar rats were randomly divided into seven groups: Control groups of A (intact), B (saline), and C (Positive control) plus test groups of D, E, F, and G (n=8). The groups D, E , and F received 6.25, 12.5, and 25 mg/kg, Borago officinalis flower hydroalcholic extract before the test, respectively but group G received 25 mg/kg borage extract and aspirin before the test. A biphasic pain was induced by injection of formalin 1%. The obtained data were analyzed by SPSS software ver. 17 employing statistical tests of Kruskal- Wallis and Mann-Whitney. The results were expressed as mean±SD. Statistical differences were considered significant at P<0.05. </div><div><b>Results:</b> The results revealed that the acute and chronic pain behavior score in test groups of D, E, F, and G significantly decreased compared to groups A and B, but this score did not show any difference compared to group C. Moreover, chronic pain behavior score in group G was significantly lower than all other groups. </div><div><b>Discussion: </b>The results indicated that Borago officinalis hydroalcoholic extract affects the acute and chronic pain behavior response in formaline test male rats. </div> Formalin test, Rat, Pain behavior, Borago officinalis 285 290 http://bcn.iums.ac.ir/browse.php?a_code=A-10-717-1&slc_lang=en&sid=1 Mohammad Reza Shahraki iranmirshekari@yahoo.com 1370031947532846005956 1370031947532846005956 No Associate professor of physiology, Department of Physiology, Faculty of Medicine, Zahedan University of Medical Sciences and Health Services, Zahedan, Iran. Mahdieh Ahmadimoghadm research.sb@acecr.ac.ir 1370031947532846005957 1370031947532846005957 Yes Local Studies & Specialty Services of Jahad-e-Daneshgahi, Sistan and Baluchistan Branch, Zahedan, Iran. Ahmad Reza Shahraki ar_shahraki@yahoo.com 1370031947532846005958 1370031947532846005958 No surgical unit, Emam Ali Hospital