Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1392
11
1
gregorian
2014
2
1
5
1
online
1
fulltext
en
Fluoxetin Upregulates Connexin 43 Expression in Astrocyte
Behavioral Neuroscience
Behavioral Neuroscience
Original
Original
<p><b>Introduction:</b> Recent studies have shown that astrocytes play major roles in normal and disease condition of the central nervous system including multiple sclerosis (MS). Molecular target therapy studies in MS have revealed that connexin-43 (Cx43) and Aquaporin-4 (AQP4) contents of astrocytes undergo expression alteration. Fluoxetine had some effects in MS patients unrelated to its known antidepressant effects. Some of fluoxetine effects were attributed to its capability of cAMP signaling pathway stimulation. This study aimed to investigate possible acute effects of fluoxetine on Cx43 and AQP4 expression in astrocyte. </p>
<div><b>Methods:</b> Astrocytoma cells were treated for 24 hours with fluoxetine (10 and 20 &mug/ml) with or without adenyl cyclase (AC) and protein kinase A (PKA) inhibition. Cx43 expression at both mRNA and protein levels and AQP4 expression at mRNA level were evaluated.
<div><b>Results:</b> Acquired results showed that fluoxetine with and without AC and PKA inhibition resulted in Cx43 up-regulation both in mRNA and protein levels, whereas AQP4 expression have not changed. </div>
<div><b>Discussion:</b> In conclusion, data showed that fluoxetine alone and in the absence of serotonin acutely up-regulated Cx43 expression in astrocytes that can be assumed in molecular target therapy of MS patients. It seems that cAMP involvement in fluoxetine effects need more researches.</div>
</div>
Astrocyte, Fluoxetine, cAMP, Connexin-43, Aquaporin-4.
74
79
http://bcn.iums.ac.ir/browse.php?a_code=A-10-1-198&slc_lang=en&sid=1
Hossein
Mostafavi
1370031947532846006484
1370031947532846006484
No
Department of Neuroscience, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran.
Mojtaba
Khaksarian
1370031947532846006485
1370031947532846006485
No
Division of Neuroscience, Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
Mohammad Taghi
Joghataei
1370031947532846006486
1370031947532846006486
No
Department of Neuroscience, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran.
Gholamreza
Hassanzadeh
1370031947532846006487
1370031947532846006487
No
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Masoud
Soleimani
1370031947532846006488
1370031947532846006488
No
Department of Hematology, Tarbiat Modares University, Tehran, Iran.
Sanaz
Eftekhari
1370031947532846006489
1370031947532846006489
No
Division of Neuroscience, Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
Mansooreh
Soleimani
1370031947532846006490
1370031947532846006490
No
Department of Hematology, Tarbiat Modares University, Tehran, Iran.
Kazem
Mousavizadeh
1370031947532846006491
1370031947532846006491
No
Division of Neuroscience, Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
Mahmoud Reza
Hadjighassem
1370031947532846006492
1370031947532846006492
Yes
Department of Neuroscience, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran.