Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1392
1
1
gregorian
2013
4
1
4
2
online
1
fulltext
en
Evaluating the Effects of Oral Morphine on Embryonic Development of Spinal Cord in Wistar Rats
Cellular and molecular Neuroscience
Cellular and molecular Neuroscience
Original
Original
<p align="left" ><p><b><font color="#221e1f" size="1"><font color="#221e1f" size="1">Introduction: </font></font><font color="#221e1f" face="Times New Roman,Times New Roman" size="1"><font color="#221e1f" face="Times New Roman,Times New Roman" size="1"><font color="#221e1f" face="Times New Roman,Times New Roman" size="1">In the present research, the effect of morphine consumption during pregnancy on the development of the embryo’s spinal cord was studied in Wistar rat. </font></font></font><b><font color="#221e1f" size="1"><font color="#221e1f" size="1"><p align="justify">Methods: </p></font></font><font color="#221e1f" face="Times New Roman,Times New Roman" size="1"><font color="#221e1f" face="Times New Roman,Times New Roman" size="1"><font color="#221e1f" face="Times New Roman,Times New Roman" size="1">Female Wistar rats (Wt: 250-300 g) were mated with males. The test group received </font></font></font><font color="#221e1f" face="Times New Roman,Times New Roman" size="1"><font color="#221e1f" face="Times New Roman,Times New Roman" size="1"><font color="#221e1f" face="Times New Roman,Times New Roman" size="1">morphine (0.01 mg/ml) in their drinking water. Pregnant rats were later killed with chloroform </font></font></font><font color="#221e1f" face="Times New Roman,Times New Roman" size="1"><font color="#221e1f" face="Times New Roman,Times New Roman" size="1"><font color="#221e1f" face="Times New Roman,Times New Roman" size="1">on the 12th, 13th and 14th days of pregnancy, and the embryos were taken out surgically. The </font></font></font><font color="#221e1f" face="Times New Roman,Times New Roman" size="1"><font color="#221e1f" face="Times New Roman,Times New Roman" size="1"><font color="#221e1f" face="Times New Roman,Times New Roman" size="1">embryos were fixed in formalin 10% for 2 weeks. Then, the weight of fixed embryos was </font></font></font><font color="#221e1f" face="Times New Roman,Times New Roman" size="1"><font color="#221e1f" face="Times New Roman,Times New Roman" size="1"><font color="#221e1f" face="Times New Roman,Times New Roman" size="1">calculated by a scale. In addition, several animals’ sizes including fronto-posterior and lateral length were measured by a caliper. Tissue processing, sectioning and hematoxylin and eosin (H&E) staining were applied for the embryos. The sections were examined for spinal cord development by light microscope and MOTIC software. </font></font></font><b><font color="#221e1f" size="1"><font color="#221e1f" size="1"><p align="justify">Results: </p></font></font><font color="#221e1f" face="Times New Roman,Times New Roman" size="1"><font color="#221e1f" face="Times New Roman,Times New Roman" size="1"><font color="#221e1f" face="Times New Roman,Times New Roman" size="1">Significant decrease was observed in the fronto-posterior and lateral length and the </font></font></font><font color="#221e1f" face="Times New Roman,Times New Roman" size="1"><font color="#221e1f" face="Times New Roman,Times New Roman" size="1"><font color="#221e1f" face="Times New Roman,Times New Roman" size="1">weight of the embryos in the test groups. The thickness of the white matter layer decreased on the 12th, 13th and 14th embryonic days. The thickness of the spine's grey layer was also less than the control group, on the same days. Increase in the length of the ependimal duct observed as well. Number of grey substance cells decreased compared to the control group within the same days. Meanwhile, thickness of the germinal layer reduced in comparison to the control group on the mentioned days. </font></font></font><b><font color="#221e1f" size="1"><font color="#221e1f" size="1"><p>Discussion: </p></font></font><font color="#221e1f" face="Times New Roman,Times New Roman" size="1"><font color="#221e1f" face="Times New Roman,Times New Roman" size="1"><font color="#221e1f" face="Times New Roman,Times New Roman" size="1">In conclusion, morphine consumption during pregnancy causes defects in growth and completion of the spinal cord. </font></font></font></b></b></b></b></p>
Spinal Cord,White Matter,Grey Matter,Epandimal Duct,Morphine,Rat.
130
135
http://bcn.iums.ac.ir/browse.php?a_code=A-10-1-152&slc_lang=en&sid=1
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A.Niknam
1370031947532846004142
1370031947532846004142
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Mahnaz
Azarnia
1370031947532846004143
1370031947532846004143
No
Hossein
Bahadoran
1370031947532846004144
1370031947532846004144
No
Masoomeh
Kazemi
1370031947532846004145
1370031947532846004145
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Elaheh
Tekieh
1370031947532846004146
1370031947532846004146
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Mina
Ranjbaran
1370031947532846004147
1370031947532846004147
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Hedayat
Sahraei
1370031947532846004148
1370031947532846004148
Yes