Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1396
12
1
gregorian
2018
3
1
0
Accepted Articles
online
1
fulltext
en
Evaluation of the Association between ABCB1, Mu-Opioid Receptor, and Cytochrome P450 Genes and Methadone Dose in Patients Undergoing Methadone Maintenance Treatment in Mazandaran Province in Iran
Clinical Neuroscience
Clinical Neuroscience
Original
Original
<div style="text-align: justify;"><span style="font-size:14px;"><span style="font-family:Tahoma;"><span style="line-height:2;"><b>Background:</b> Treatment of opioid use disorders via safe and effective approaches has been investigated for years, from the past to the present. Methadone maintenance treatment (MMT) has been considered an effective therapy for opioid addiction. It has been observed that patients with genetic polymorphisms often show variability in the optimal drug dose requirement and treatment schedule. This study aimed to investigate the relationship between single nucleotide polymorphisms (SNPs) in the ATP-binding cassette subfamily B member-1 (ABCB1), the mu-opioid receptor (OPRM1)<span style="background:white">, and </span>cytochrome P450 (CYP)<span style="background:white"> genes and methadone dose </span>in patients undergoing MMT in Mazandaran province in Iran<span style="background:white">.</span><br>
<b><span style="background:white">Methods:</span></b><span style="background:white"> I</span>n a cross-sectional study, 216 male MMT patients (20 to 45 years old) who were involved in the treatment program for at least three months were randomly recruited from six MMT clinics in Mazandaran province between 2018 and 2020. Blood samples were taken, DNA was extracted, and SNPs of CYP2B6 (G516T, A785G), CYP2C19 (-3402C>T), CYP3A4 (-392A>G), OPRM1 )A118G), and ABCB1 (C3435T, G2677T, G2677A, and C1236T) <span style="background:white">genes were evaluated using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). </span><br>
<b><span style="background:white">Results:</span></b> Our results showed no significant relationship between all the studied genotypes and methadone dose requirements.<br>
<b>Conclusion:</b> The present study, for the first time in the Mazandaran population, reported no significant correlations between methadone dose requirement and different SNPs in the ABCB1, <span style="background:white">OPRM1, and CYP genes</span> in MMT patients, which is consistent with other studies conducted on the Iranian population. </span></span></span></div>
Methadone maintenance, ABCB1, OPRM1, CYP, Addiction, Iran, PCR-RFLP
0
0
http://bcn.iums.ac.ir/browse.php?a_code=A-10-2756-2&slc_lang=en&sid=1
Abdollah
Golnezhad
13700319475328460043886
13700319475328460043886
No
Department of Neuroscience and Addiction Studies, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Anahita
Torkaman-Boutorabi
13700319475328460043887
13700319475328460043887
No
Department of Neuroscience and Addiction Studies, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.; Research Center for Cognitive and Behavioral Sciences, Tehran University of Medical Sciences, Tehran, Iran.
Emran Mohammad
Razaghi
13700319475328460043888
13700319475328460043888
No
Department of Psychiatry, Tehran University of Medical Sciences, Tehran, Iran.
Mohammad Reza
Zarrindast
13700319475328460043889
13700319475328460043889
No
Department of Neuroscience and Addiction Studies, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.; Iranian National Center for Addiction Studies, Tehran University of Medical Sciences, Tehran, Iran.
Mohammad Reza
Mahdavi
13700319475328460043890
13700319475328460043890
No
Thalassemia Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran.
Nasim
Vousooghi
n-vousooghi@tums.ac.ir
13700319475328460043891
13700319475328460043891
Yes
Research Center for Cognitive and Behavioral Sciences, Tehran University of Medical Sciences, Tehran, Iran.; Iranian National Center for Addiction Studies, Tehran University of Medical Sciences, Tehran, Iran.; Department of Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.