Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1396
12
1
gregorian
2018
3
1
0
Accepted Articles
online
1
fulltext
en
The Effects of Exogenous IGF-I and MGF on Neural Stem Cells Proliferation in Hypoxic Environments in Vitro
Cellular and molecular Neuroscience
Cellular and molecular Neuroscience
Original
Original
<h1 style="text-align: justify;"><span style="font-size:20pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="font-size:12.0pt"><span style="line-height:150%"><span style="font-weight:normal">Ischemic stroke has high </span></span></span><span style="font-size:12.0pt"><span style="line-height:150%"><span style="font-weight:normal">morbidity and mortality rates worldwide. L</span></span></span><span style="font-size:12.0pt"><span style="line-height:150%"><span style="font-weight:normal">ow oxygen (O<sub>2</sub>) levels detected in such conditions create a vulnerable environment for neural stem cells (NSC)<span style="background:white">, altering neuronal function and leading to neuronal injury or death</span>. </span></span></span><span style="font-size:12.0pt"><span style="line-height:150%"><span style="font-weight:normal">There are still no effective treatments for such consequences. We have investigated the molecular and functional effects of growth factors, IGF-I and MGF in NSC exposed to low </span></span></span><span style="font-size:12.0pt"><span style="line-height:150%"><span style="font-weight:normal">O<sub>2 </sub></span></span></span><span style="font-size:12.0pt"><span style="line-height:150%"><span style="font-weight:normal">levels. </span></span></span><span style="font-size:12.0pt"><span style="line-height:150%"><span style="font-weight:normal">An</span></span></span><span style="font-size:12.0pt"><span style="line-height:150%"><span style="font-weight:normal"> <i>in vitro</i> ischemia model was created by rat hippocampal NSC grown in culture that were exposed to varying oxygen levels including 0%, 3%, and 20 % for the representation of anoxic, hypoxic, and normoxic conditions, respectively, during the 24 hours. NSC<i> </i>was investigated for <i>IGF-I</i>, <i>MGF</i>, and<i> </i></span></span></span><i><span style="font-size:12.0pt"><span style="line-height:150%"><span style="font-weight:normal">HIF1</span></span></span></i><i><span style="font-size:12.0pt"><span style="line-height:150%"><span style="font-weight:normal">-</span></span></span></i><i><span style="font-size:12.0pt"><span style="line-height:150%"><span style="font-weight:normal">A</span></span></span></i><i><span style="font-size:12.0pt"><span style="line-height:150%"><span style="font-weight:normal">lpha</span></span></span></i> <span style="font-size:12.0pt"><span style="line-height:150%"><span style="font-weight:normal">(<i>HIF-1α</i>)</span></span></span> <span style="font-size:12.0pt"><span style="line-height:150%"><span style="font-weight:normal">gene expressions by real-time RT-PCR. The effects of external administration of growth factors, IGF-I and MGF on NSC proliferation in such conditions were explored. </span></span></span><span style="font-size:12.0pt"><span style="line-height:150%"><span style="font-weight:normal">Increased <i>IGF-I</i> and <i>MGF</i> gene expressions were detected in the samples exposed to low O<sub>2</sub>. Anoxia was the highest stimulant for <i>IGF-I</i> and <i>MGF </i>expressions. <i>HIF1-</i>α that encodes hypoxia-inducible factor-1α revealed downregulation in relative gene expression fold change with IGF-I application in all conditions, whereas MGF application upregulated its change in an anoxic environment. Furthermore, MGF-induced NSC had more proliferation- migration rate in all oxygen conditions. IGF-I induced significant NSC proliferation in 0% and 20% O<sub>2</sub>. </span></span></span><span style="font-size:12.0pt"><span style="line-height:150%"><span style="font-weight:normal">These findings suggest that <i>IGF-I</i> and <i>MGF</i> expressions were increased to reduce the damage in NSC exposed to low oxygen, and exogenous MGF and IGF-I application increased NSC proliferation at the time of injury. The results might imply the role of exogenous MGF and IGF-I in the treatment of ischemia for relieving the effect of neuronal damage due to their neuroprotective and proliferative effects.</span></span></span></span></span></span></h1>
Neural Stem Cell, Hypoxia, Growth Factors, MGF, IGF-I
0
0
http://bcn.iums.ac.ir/browse.php?a_code=A-10-3981-1&slc_lang=en&sid=1
Tuğba
Aydıntuğ-Gürbüz
tbaydintug@gmail.com
13700319475328460042101
13700319475328460042101
No
Department of Genetics, Aziz Sancar Research Institute of Experimental Medicine, Istanbul University
Fatih
Toprak
drfatihtoprak@gmail.com
13700319475328460042102
13700319475328460042102
No
Department of Neurosurgery, Haydarpaşa Numune Training and Research Hospital
Selin
Toprak
tprkselin@gmail.com
13700319475328460042103
13700319475328460042103
No
Department of Genetics, Aziz Sancar Research Institute of Experimental Medicine, Istanbul University
Selçuk
Sözer
ssozer@istanbul.edu.tr
13700319475328460042104
13700319475328460042104
Yes
Department of Genetics, Aziz Sancar Research Institute of Experimental Medicine, Istanbul University