Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1396
12
1
gregorian
2018
3
1
0
Accepted Articles
online
1
fulltext
en
A Novel Approach for Mucosal and Bulbar Olfactory Ensheathing Cell Isolation Based on the Non-Adherent Subculture Technique
Cellular and molecular Neuroscience
Cellular and molecular Neuroscience
Original
Original
<div style="text-align: justify;"><span style="font-size:12px;"><span style="line-height:150%"><span sans-serif=""><span style="line-height:150%"><span new="" roman="" times="">Olfactory ensheathing cells (OECs) are widely used in transplantation studies. Therefore, the high purification of this unique cell type will be valuable for medical applications. Although recent improvements in OEC isolation procedures opened a new era in this field, the high purification efficacy and viability rate are still needed to be considered. The most widely used OECs isolation techniques can be broadly classified as based on adherence properties in particular in the case of olfactory bulb-derived OECs isolation. Considering the invasive nature of harvesting OECs from human olfactory bulbs, hence the high efficient purification of these cells from olfactory mucosa can be beneficial in clinical trials. In this study, we isolated OECs from both the olfactory bulb and mucosa of rats due to their differential adherence properties and compared them. Cell preparations were characterized by NGFR p75 and S100β antibodies, the specific markers for OECs using immunocytochemistry and western blot analysis respectively. OECs morphology and viability were monitored over time by microscopy and MTT assay. We found that utilizing our suggested method, OECs could be purified from the olfactory mucosa as efficiently as the olfactory bulb. Both sources-derived OECs showed high levels of NGFR p75 and S100β expression, although the S100β expression was higher in olfactory mucosa-derived OECs preparations (</span></span><span lang="EN"><span style="line-height:150%"><span new="" roman="" times="">P<0.05)</span></span></span><span style="line-height:150%"><span new="" roman="" times="">. Moreover, there was no significant difference between the two sources in cell viability in our suggested protocol. Hence due to the non-invasive harvesting method, olfactory mucosa-derived OECs are preferred from a clinical point of view for transplantation studies.</span></span></span></span></span></div>
Olfactory ensheathing cells, Cell purification, Cell transplantation, Olfactory bulb, Olfactory mucosa
0
0
http://bcn.iums.ac.ir/browse.php?a_code=A-10-3579-1&slc_lang=en&sid=1
Fatemeh
Tirgar
serah086@yahoo.com
13700319475328460040315
13700319475328460040315
No
PhD Student Department of Neuroscience and Addiction Studies, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Zahra
Azizi
zahra.azizivarzaneh@gmail.com
13700319475328460040316
13700319475328460040316
No
Assistant Professor Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Mahmoudreza
Hadjighassem
mhadjighassem@tums.ac.ir
13700319475328460040317
13700319475328460040317
Yes
Associate Professor Department of Neuroscience and Addiction Studies, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.