en Cellular and molecular Neuroscience Cellular and molecular Neuroscience Original Original <div style="text-align: justify;"><strong>Introduction</strong>: Schizophrenia (SCZ) is a psychiatric disorder caused by environmental, social, and genetic factors. This phenomenon is a severe neuropsychiatric disorder with a 1% worldwide prevalence. As SCZ is an exclusively human disorder, animal models cannot mimic SCZ pathophysiology. Thus, it is crucial to develop a novel human-based specific model of SCZ to elucidate mechanisms of the occurrence of the disease. In this regard, reprogramming somatic cells to human-induced pluripotent stem cells (hiPSCs) serves as an expensive instrument for modeling SCZ.&nbsp;<br> <strong>Methods</strong>: In the present study, we directly reprogrammed the isolated human ear dermal fibroblasts (HDFs) from schizophrenic patients into hiPSCs using some episomal agents in Matrigel-coated plates. The existence of pluripotency markers was confirmed by the immunocytochemistry (ICC) test and alkaline phosphatase protocol. We performed karyotype analysis to ensure the maintenance of the normal chromosomes.&nbsp;<br> <strong>Results</strong>: Analysis of colonies exhibited intense alkaline phosphatase engagement and Oct4, SSEA4, Nanog, and Tra-1-60. HiPSCs showed normal karyotypes and were potent to differentiate into ectoderm, endoderm, and mesoderm.&nbsp;<br> <strong>Conclusion</strong>: Application of hiPSCs derived from schizophrenic patients would be a promising approach to treating SCZ. For checking the behavior of the cells during neurogenesis, we suggest further studies be applied.</div> Schizophrenia, hiPSCs, Fibroblast, Episomal vectors 333 342 http://bcn.iums.ac.ir/browse.php?a_code=A-10-3697-1&slc_lang=en&sid=1 Asghar Parvishan omid_north@yahoo.com 13700319475328460049492 0009-0008-1206-6492 No Department of Neuroscience, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran. Mohammad Taghi Joghataei joghataei@iums.ac.ir 13700319475328460049493 0009-0005-6748-6468 Yes Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran. Jafar Kiani ja.kiani@gmail.com 13700319475328460049494 13700319475328460049494 No Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran. Ali Shahbazi shahbazial@yahoo.com 13700319475328460049495 13700319475328460049495 No Department of Neuroscience, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran. Faezeh Faghihi faezefaghihi@yahoo.com 13700319475328460049496 13700319475328460049496 No Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran. Mohammad Ghadiri 13700319475328460049497 13700319475328460049497 No Psychiatry Center of Iran, Iran University of Medical Sciences, Tehran, Iran.