en Cellular and molecular Neuroscience Cellular and molecular Neuroscience Original Original <div style="text-align: justify;"><span style="font-size:14px;"><span style="font-family:Tahoma;"><span style="line-height:2;"><strong>Introduction</strong>: In the present study, the culture of embryonic spinal motor neurons (SMNs) was used to assess the impacts of adrenomedullin (AM) on the neurotoxic effects of doxorubicin (DOX).<br> <strong>Methods</strong>: To prepare the culture of rat embryonic SMNs, spinal cords were isolated from the rat embryos, digested enzymatically, and triturated to obtain spinal cell suspension. Then, the SMNs were purified from the cell suspension using a single OptiPrep gradient and cultured. The SMNs were treated with DOX (0.0-100 &micro;M) and AM (3.125-100 nM), and their viability and apoptosis were evaluated using MTT and annexin V flow cytometric assays. Oxidative stress was assessed through the measurement of cellular reactive oxygen species (ROS), nitric oxide (NO), malondialdehyde (MDA), and 8-iso-prostaglandin F2&alpha; (iPF2&alpha;) levels. Finally, qPCR was employed to determine the expressions of interleukin1-&beta; (IL-1&beta;), inducible NO synthase (iNOS), tumor necrosis factor-&alpha; (TNF-&alpha;), SRY-related protein 9 (SOX9), matrix metalloproteinase (MMP)-3 and -13.&nbsp;<br> <strong>Results</strong>: The viability of SMNs decreased following DOX treatment dose-dependently (IC50=10.54 &micro;M). DOX increased the cellular ROS, MDA, NO, and iPF2&alpha; levels (P<0.001). Additionally, AM reduced DOX-induced cell death dose-dependently (P<0.001). AM (50 nM) pretreatment also reduced the DOX-induced oxidative stress (P<0.01) and gene expression (P<0.01).&nbsp;<br> <strong>Conclusion</strong>: Based on the results, AM might be a protective factor against chemotherapy-induced toxicity in SMNs.&nbsp;</span></span></span></div> Spinal motor neuron (SMN), Doxorubicin (DOX), Adrenomedullin (AM), Oxidative stress, Inflammation 617 630 http://bcn.iums.ac.ir/browse.php?a_code=A-10-3650-1&slc_lang=en&sid=1 Mohammad Ali Takhshid takhshidma@sums.ac.ir 13700319475328460050671 13700319475328460050671 Yes Division of Medical Biotechnology, Department of Laboratory Sciences, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran. Amir Mahmoodazdeh Amir.biochem@gmail.com 13700319475328460050672 13700319475328460050672 No Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Sayed Mohammad Shafiee shafieem@sums.ac.ir 13700319475328460050673 13700319475328460050673 No Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Mohsen Sisakht mohsen.sisakht@gmail.com 13700319475328460050674 13700319475328460050674 No Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Zahra khosdel Khoshdelz@sums.ac.ir 13700319475328460050675 13700319475328460050675 No Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.