Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1401
10
1
gregorian
2023
1
1
14
1
online
1
fulltext
en
Human Olfactory Ecto-mesenchymal Stem Cells Displaying Schwann-cell-like Phenotypes and Promoting Neurite Outgrowth in Vitro
Cellular and molecular Neuroscience
Cellular and molecular Neuroscience
Original
Original
<div style="text-align: justify;"><span style="font-size:14px;"><span style="font-family:Tahoma;"><span style="line-height:2;"><strong>Introduction</strong>: Strategies of Schwann cell (SC) transplantation for regeneration of peripheral nerve injury involve many limitations. Stem cells can be used as alternative cell source for differentiation into Schwann cells. Given the high potential of neural crest-derived stem cells for the generation of multiple cell lineages, in this research, we considered whether olfactory ectomesenchymal stem cells (OE-MSCs) derived from neural crest can spontaneously differentiate into SC lineage<br>
<strong>Methods</strong>: OE-MSCs were isolated from human nasal mucosa and characterized by the mesenchymal and neural crest markers. The cells were cultured in glial growth factors-free medium and further investigated in terms of the phenotypic and functional properties. <br>
<strong>Results</strong>: Immunocytochemical staining and real-time PCR analysis indicated that the cultured OE-MSCs expressed SCs markers, SOX10, p75, S100, GFAP and MBP, differentiation indicative. It was found that the cells could secrete neurotrophic factors, including brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF). Furthermore, after co-cultured with PC12, the mean neurite length was enhanced by OE-MSCs. <br>
<strong>Conclusion</strong>: The findings indicated that OE-MSCs could be differentiated spontaneously into SC-like phenotypes, suggesting their applications for transplantation in peripheral nerve injuries.</span></span></span></div>
Spontaneous differentiation, Olfactory ecto-mesenchymal stem cells, Schwann cell, Peripheral nerve regeneration
31
42
http://bcn.iums.ac.ir/browse.php?a_code=A-10-3542-1&slc_lang=en&sid=1
Maedeh
Entezari
entezarinm@gmail.com
13700319475328460044411
13700319475328460044411
No
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran. ; Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Mehrdad
Bakhtiari
mehr_bakhtiyari@yahoo.com
13700319475328460044412
13700319475328460044412
No
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran. ; Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Fatemeh
Moradi
f7moradi@gmail.com
13700319475328460044413
13700319475328460044413
No
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran. ; Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Masoud
Mozafari
mozafari.masoud@gmail.com
13700319475328460044414
13700319475328460044414
No
Department of Tissue Engineering & Regenerative Medicine, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
Zohreh
Bagher
baharebagher@gmail.com
13700319475328460044415
13700319475328460044415
No
Department of Tissue Engineering & Regenerative Medicine, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran. ; ENT and Head and Neck Research Center and Department, The Five Senses Health Institute, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Mansoureh
Soleimani
mansourehsoleimani@gmail.com
13700319475328460044416
13700319475328460044416
Yes
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran. ; Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.