Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1401
6
1
gregorian
2022
9
1
13
5
online
1
fulltext
en
Cannabidiol Modulating the Expression of Neurotrophin Signaling Pathways in Chronic Exposure to Methamphetamine in Rats During Abstinence Period
Behavioral Neuroscience
Behavioral Neuroscience
Original
Original
<strong>Introduction: </strong>Several neuropsychiatric disorders, such as addiction, have indicated variations in the levels of neurotrophic factors. As an extremely addictive stimulant, methamphetamine (METH) is associated with rising levels of abuse worldwide. We have recently demonstrated that repeated intracerebroventricular (ICV) of cannabidiol (CBD), the most important non-psychotomimetic compound, can lead to diminished impairing memory and hippocampal damage caused by chronic exposure to METH (CEM) in rats over the abstinence period. Furthermore, the results indicated a possible contribution of the neurotrophin signaling pathway (NSP) in regulating neurogenesis and survival. This study intends to evaluate whether these effects remained as measured in molecular pathways after the abstinence period.<br>
<strong>Methods:</strong> The animals were given 2mg/kg METH twice a day for 10 days. Then, we adopted real-time polymerase chain reaction (PCR) throughout the 10-day abstinence period to assess the CBD’s effect (10 and 50μg/5μL) on the levels of the mRNA expression of the NSP.<br>
<strong>Results: </strong>The findings suggested that CEM, when compared to the control group in the hippocampus, downregulated mRNA expression of NSP. Moreover, a dosage of 50 μg/5μL CBD may possibly enhance the mRNA expression level of BDNF/TrkB and NGF/TrkA in the hippocampus. Besides, the expression of RAF-1 mRNA level could be reversed significantly by both doses of CBD.<br>
<strong>Conclusion:</strong> According to our results, CBD may partly bring about neuroprotective effects by modulating the NSP. These findings set forth solid evidence demonstrating that CBD is a protective factor attributed to neuropsychiatric disorders, such as METH addiction.
Methamphetamine, Cannabidiol, Neurotrophin signaling pathway, Chronic exposure, Abstinence, Hippocampus
719
730
http://bcn.iums.ac.ir/browse.php?a_code=A-10-3059-1&slc_lang=en&sid=1
Yasaman
Razavi
Razavi.yasaman@gmail.com
13700319475328460042815
13700319475328460042815
No
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
Mohammad
Najafi
nbsmmsbn@iums.ac.ir
13700319475328460042816
13700319475328460042816
No
Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Abbas
Haghparast
Haghparast@yahoo.com
13700319475328460042817
13700319475328460042817
No
Neuroscience Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Fariborz
Keyhanfar
keyhanfar.f@iums.ac.ir
13700319475328460042818
13700319475328460042818
No
Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Ronak
Shabani
Shabani.r@iums.ac.ir
13700319475328460042819
13700319475328460042819
No
Reproductive Sciences and Technology Research Center, Department of Anatomy, Iran University of Medical Sciences, Tehran, Iran.
Mehdi
Mehdizadeh
mehdizadeh.m@iums.ac.ir
13700319475328460042820
13700319475328460042820
Yes
Reproductive Sciences and Technology Research Center, Department of Anatomy, Iran University of Medical Sciences, Tehran, Iran.