en Behavioral Neuroscience Behavioral Neuroscience Original Original <span style="font-size:14px;"><span style="font-family:Tahoma;"><span style="line-height:200%"><span style="background:white"><span style="line-height:200%"><span style="color:#252525">This study evaluates the effects of hydro-alcoholic root extract of <i>Delphinium denudatum W</i>all (Jadwar, family; Ranunculaceae) for its ability to attenuate the nicotine-withdrawal in nicotine-dependent rats. &nbsp;The physical dependence on nicotine was induced in male adult Wistar albino rats (175-250 g)&nbsp;&nbsp; by subcutaneous implantation of Alzet mini osmotic pumps which supplied nicotine at 9.0mg/kg/day, while control rats received saline via osmotic pumps. For seven days four separate test doses of hydro-alcoholic root extract of <i>Delphinium denudatum Wall </i>(200, 400, 800, 1600 mg/kg) were given orally. &nbsp;On the 7^th&nbsp; day, a mecamylamine injection (1 mg/kg, s.c.) was given to precipitate nicotine-withdrawal.</span></span><span style="color:#252525"> The global&nbsp; Gellert&rsquo;s&ndash;Holtzman rating scale was used to rate somatic signs of withdrawal for 15 minutes, followed by a measurement of motor activity.&nbsp; Drug Bupropion was used as a positive control. Serum levels of nicotine (cotinine) and corticosterone were done by ELISA.&nbsp; In nicotine-dependent rats, oral administration of <i>Delphinium denudatum Wall</i> root extract suppressed the hyper-locomotion and decreased the corticosterone levels at all dosages.&nbsp; Higher doses (800 and 1600 mg/kg) of extract,&nbsp;&nbsp; significantly attenuated nicotine withdrawal whereas,&nbsp; lower doses (200 and 400 mg/kg) had no significant effect. These results suggest that hydro-alcoholic root extract of Delphinium denudatum Wall may prove to be a potential therapeutic agent to attenuate nicotine dependence and facilitate tobacco smoking </span></span><span style="line-height:200%">cessation.</span></span></span></span><br> <br> <gdiv></gdiv> Delphinium denudatum Wall, Nicotine, Hydro-alcoholic, Withdrawal, Bupropion, Mecamylamine 0 0 http://bcn.iums.ac.ir/browse.php?a_code=A-10-2559-1&slc_lang=en&sid=1 Raka Jain rakajain2009@gmail.com 13700319475328460045782 13700319475328460045782 Yes National Drug Dependence Treatment Centre, Department of Psychiatry, All India Institute of Medical Sciences, New Delhi, India. Rahul Raghav raghavrahul74@gmail.com 13700319475328460045783 13700319475328460045783 No National Drug Dependence Treatment Centre, Department of Psychiatry, All India Institute of Medical Sciences, New Delhi, India. Mohd. Waseem wasmsss@gmail.com 13700319475328460045784 13700319475328460045784 No National Drug Dependence Treatment Centre, Department of Psychiatry, All India Institute of Medical Sciences, New Delhi, India. Azhar Jabin ajabin@jamiahamdard.ac.in 13700319475328460045785 13700319475328460045785 No Faculty of Unani Medicine, Jamia Hamdard University, New Delhi, India. Sonali Jhanjee sonalijhanjee@gmail.com 13700319475328460045786 13700319475328460045786 No National Drug Dependence Treatment Centre, Department of Psychiatry, All India Institute of Medical Sciences, New Delhi, India.