Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1399
10
1
gregorian
2021
1
1
12
1
online
1
fulltext
en
Comparative Study of CRH Microinjections into PVN and CeA Nuclei on Food Intake, Ghrelin, Leptin, and Glucose Levels in Acute Stressed Rats
Behavioral Neuroscience
Behavioral Neuroscience
Original
Original
<strong>Introduction</strong>: Corticotropin-Releasing Hormone (CRH) is involved in stress and energy homeostasis. On the other hand, CRH receptors also exist within the paraventricular nucleus (PVN) and Central Amygdala (CeA) nuclei. The present study compared the effect of CRH microinjections into PVN and CeA on three consecutive hours and cumulative food intake, internal regulatory factors of food intake, such as serum leptin and ghrelin, as well as blood glucose levels in rats under different acute psychological (Social Stress [SS] and Isolation Stress [IS] group) stresses.<br>
<strong>Methods</strong>: Sixty-six male Wistar rats were randomly allocated to 11 groups: Control, Sham, CRH-PVN, CRH-CeA, SS, IS, SS-CRH-PVN, SS-CRH-CeA, IS-CRH-PVN, and IS-CRH-CeA groups. The CRH (2 µg/kg in 0.5 µL saline) was injected into PVN and CeA nuclei in rats under everyday, acute social stress and isolation stress conditions. <br>
<strong>Results</strong>: Acute isolation and social stresses did not affect cumulative food intake. Whereas isolation stress led to changes in both leptin and glucose levels, social stress reduced only glucose levels. Cumulative food intake significantly decreased under acute CRH injection into the CeA and particularly into the PVN. Blood glucose significantly reduced in all the groups receiving CRH into their CeA. <br>
<strong>Conclusion</strong>: The PVN played a more important role compared to CeA on food intake. These nuclei probably employ different mechanisms for their effects on food intake. Besides, it seems that exogenously CRH injection into the PVN probably had a more anorectic effect than naturally activated CRH by stresses. Acute isolation stress had a greater impact than social stress on leptin level and cumulative food intake. Thus, elevated food intake related to leptin compared to ghrelin and glucose levels in the CRH-PVN group under acute social stress.
Corticotropin-releasing hormone, Paraventricular, Central amygdala, Social stress, Isolation stress, Food intake
133
148
http://bcn.iums.ac.ir/browse.php?a_code=A-10-2346-1&slc_lang=en&sid=1
Maryam
Radahmadi
m_radahmadi@med.mui.ac.ir
13700319475328460031605
13700319475328460031605
Yes
Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Mina Sadat
Izadi
mina.izadi72@gmail.com
13700319475328460031606
13700319475328460031606
No
Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Atefeh
Rayatpour
atefeh_rayatpour@yahoo.com
13700319475328460031607
13700319475328460031607
No
Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Maedeh
Ghasemi
ghasemi.m.ph@gmail.com
13700319475328460031608
13700319475328460031608
No
Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.