en Cellular and molecular Neuroscience Cellular and molecular Neuroscience Original Original <strong>Introduction</strong>: The present study addressed whether methylprednisolone (MP) as an anti-inflammatory drug used in neurodegenerative diseases and neural stem/progenitor cells (NS/PCs) is safe.&nbsp;<br> <strong>Methods</strong>: First, embryonic rat NS/PCs were exposed to different concentrations of MP, and then we evaluated their survival by MTT assay, proliferation by analyzing the number and diameter of neurospheres, and the migration of the cells by neurosphere assay.<br> <strong>Results</strong>: The viability of NS/PCs was reduced following exposure to 10, 15, and 20 &micro;g/mL of MP. In addition, although the number of neurospheres did not change, exposure to different concentrations of MP resulted in the formation of smaller neurospheres. Despite these undesirable effects, the highest concentration of MP (20 &mu;g/mL) increased the migration capacity of the NS/PCs.<br> <strong>Conclusion</strong>: The combination of MP and NS/PCs is not recommended due to the adverse effects of MP on the survival and proliferation of NS/PCs.<br> <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:200%"><span style="font-family:Calibri,sans-serif"><span style="font-size:12.0pt"><span style="line-height:200%"></span></span></span></span></span></span> Methylprednisolone, Neural stem cells, Proliferation, Survival, Migration 207 214 http://bcn.iums.ac.ir/browse.php?a_code=A-10-35-4&slc_lang=en&sid=1 Zohreh Bagheri 13700319475328460040666 13700319475328460040666 No Department of Physiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Fatemeh Shamsi f.shamsi86@yahoo.com 13700319475328460040667 13700319475328460040667 No Department of Neurosciences, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran. Zahra Zeraatpisheh zahrazeraatpisheh@yahoo.com 13700319475328460040668 13700319475328460040668 No Department of Neurosciences, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran. Mahin Salmannejad 13700319475328460040669 13700319475328460040669 No Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Ahmad Soltani 13700319475328460040670 13700319475328460040670 No Department of Neurosurgery, Namazi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran. Hadi Aligholi aligholi@sums.ac.ir 13700319475328460040671 13700319475328460040671 Yes Department of Neurosciences, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.