en Cellular and molecular Neuroscience Cellular and molecular Neuroscience <strong>Introduction</strong>: Multiple Sclerosis (MS) is the chronic inflammation of the Central Nervous System (CNS) and autoimmune disease. MS is most widely considered to be mediated by the activation of myelin-specific T CD4+ cells as well as TH1 and TH17 cells. TH17 cells are involved in the pathogenesis of MS in various manners. HIF-1&alpha; and RORC are required for the natural differentiation of TH17; they are essential transcription factors for the evolution of TH17 cells. Numerous studies indicated that Epigallocatechin Gallate (EGCG) presents immunomodulatory and anti-inflammatory effects. This study investigated the effects of EGCG on normoxic HIF-1&alpha; and RORC2 expression in PBMCs among MS patients.<br> <strong>Methods</strong>: Peripheral Blood Mononuclear Cells (PBMCs) were isolated from the whole blood of new cases of MS. The cells were cultured in the presence of a different concentration of EGCG (25, 50,100&mu;M) for 18 and 48 hours. Next, HIF-1&alpha; and RORC2 level expressions were measured by Enzyme-Linked Immunosorbent Assay (ELISA) and Real-Time PCR, respectively.&nbsp;<br> <strong>Results</strong>: The results showed that EGCG significantly decreased RORC2 gene expression. EGCG did not affect the level of HIF-1&alpha;.&nbsp;<br> <strong>Conclusion</strong>: However, EGCG did not influence the level of HIF-1&alpha;. Our present data has led us to conclude that EGCG could be considered as an anti-inflammatory agent may serve as an achievable therapeutic agent for MS. Multiple Sclerosis, Epigallocatechin-3-Gallate (EGCG), RORC2, Hypoxia-Inducible Factor 1-Alpha (HIF-1α) 533 540 http://bcn.iums.ac.ir/browse.php?a_code=A-10-2252-1&slc_lang=en&sid=1 Boshra Afshar Boshraafshar@yahoo.com 13700319475328460037995 13700319475328460037995 No Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Mazdak Ganjalikhani-Hakemi mghakemi@med.mui.ac.ir 13700319475328460037996 13700319475328460037996 No Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Zahra Khalifezadeh Esfahani Zahra.khz@yahoo.com 13700319475328460037997 13700319475328460037997 No Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Nahid Eskandari neskandari@med.mui.ac.ir 13700319475328460037998 13700319475328460037998 No Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Vahid Shaygannajad Shaygannejad@med.mui.ac.ir 13700319475328460037999 13700319475328460037999 No Department of Neurology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Fahimeh Hosseininasab fahim60.h@gmail.com 13700319475328460038000 13700319475328460038000 No Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Freshteh Alsahebfosoul alsahebfosoul@med.mui.ac.ir 13700319475328460038001 13700319475328460038001 Yes Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.