Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1400
10
1
gregorian
2022
1
1
13
1
online
1
fulltext
en
Oral Administration of Probiotic Enterococcus durans to Ameliorate Experimental Autoimmune Encephalomyelitis in Mice
Cellular and molecular Neuroscience
Cellular and molecular Neuroscience
Original
Original
<div style="text-align: justify;"><strong>Introduction:</strong> Probiotics, including lactobacilli, have immunomodulatory activities with promising effects on inflammatory diseases. In this study, we evaluate the effect of Enterococcus durans (Edu) and three various strains of lactobacilli (Lacto-mix), including L. rhamnosus, L. casei, and L. plantarum, to prevent Experimental Autoimmune Encephalomyelitis (EAE) features<br>
<strong>Methods:</strong> C57BL/6 female mice were inoculated with Myelin Oigodendrocyte Glycoprotein (MOG35-55) in CFA (complete Freund’s adjuvant) to induce EAE. Five groups (n=6 in each group) of animals received saline or probiotics by oral gavage with 200 µL of lactobacilli (1.5×108 CFU/mL) for 2 weeks before the immunization and during the test for one month.<br>
<strong>Results:</strong> Histopathological studies showed an increase in infiltration of inflammatory cells and destruction of the myelin membrane in the EAE group but a decrease in inflammatory cells in the probiotic-treated animals. Pro-inflammatory cytokines (Interleukin [IL]-17 and Interferon [IFN]-γ) concentration in the supernatant of the brain and spinal cord tissues showed a significant increase in the EAE compared with the normal saline group (P<0.01). While in the spinal cord tissue, there was a decrease in IL-17 in those animals treated with the Lacto-mix and Edu + Lacto-mix (P<0.01) and a significant decrease in IFN-γ in those animals that received Edu (P<0.05). Western blot analysis of matrix metalloproteinase-9 and myelin basic protein showed a decrease and increase in treatment and EAE groups, respectively, compared to the normal control group. <br>
<strong>Conclusion:</strong> Our data suggest that probiotic Enterococcus durans and Lacto-mix prevents EAE, but further studies are needed to clarify the exact mechanisms and their application in preclinical and clinical trials.</div>
Multiple Sclerosis (MS), Experimental autoimmune encephalomyelitis, Anti-inflammatory, Antioxidant, Probiotic, Enterococcus durans
35
46
http://bcn.iums.ac.ir/browse.php?a_code=A-10-1955-1&slc_lang=en&sid=1
Seyed Abdollah
Samani
samani1352@yahoo.com
13700319475328460040433
13700319475328460040433
No
Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Mohammad Raman
Moloudi
X.moloudi@muk.ac.ir
13700319475328460040434
13700319475328460040434
No
Liver and Digestive Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Rashid
Ramezanzadeh
atrop_t51@yahoo.com
13700319475328460040435
13700319475328460040435
No
Department of Microbiology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Mohammad
Abdi
Abdi@muk.ac.ir
13700319475328460040436
13700319475328460040436
No
Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Bahram
Nikkhoo
bahram.nikkhoo@muk.ac.ir
13700319475328460040437
13700319475328460040437
No
Department of Pathology and Clinical Laboratory Sciences, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Esmael
Izadpanah
ezadpanah2000@gmail.com
13700319475328460040438
13700319475328460040438
No
Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Daem
Roshani
Daemroshani@gmail.com
13700319475328460040439
13700319475328460040439
No
Department of Epidemiology and Biostatistics, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Alina
Abdolahi
alina.abdolahi@yahoo.com
13700319475328460040440
13700319475328460040440
No
Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Parisa
Esmaili
parisaesmaili27@yahoo.com
13700319475328460040441
13700319475328460040441
No
Department of Immunology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Kambiz
Hassanzadeh
kambizhassanzadeh@gmail.com
13700319475328460040442
13700319475328460040442
Yes
Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.