en Behavioral Neuroscience Behavioral Neuroscience Original Original <div style="text-align: justify;"><strong>Introduction</strong>: The present study aimed to investigate the antidepressant effect of Verbena (V.) officinalis L. aqueous extract in adult female rats.&nbsp;<br> <strong>Methods</strong>: The present study evaluated the antidepressant effect of V. officinalis L. aqueous (V AE) extract in female rats using the Forced-Swimming Test (FST), Light-Dark Box (LDB) test, and Open Field Test (OFT). The level of glycemia and histological analysis were also studied. The VAE [200 mg/kg Parenterally (PO)] was administered orally for 7 successive days in the separate groups of rats.&nbsp;<br> <strong>Results</strong>: The oral administration of V. officinalis L. aqueous extract significantly decreased (P< 0.01) the immobility time in the FST, increased the time spent in the light area (LDB), and the number of entry into the central squares (OFT). Thus, the extract at a dose of 200 mg/kg significantly decreased the glycemia level (P<0.05) and suggested no harmful effect on brain histology.<br> <strong>Conclusion</strong>: Verbena officinalis L. aqueous extract at a dose of 200 mg/kg could have an anti-depressant effect in adult rats.&nbsp;</div> Aqueous extract, Depression, Neurobehavioral tests, Verbena officinalis L 91 98 http://bcn.iums.ac.ir/browse.php?a_code=A-10-1790-1&slc_lang=en&sid=1 Amina Bekara a.bekara@univ-chlef.dz 13700319475328460025428 13700319475328460025428 Yes Laboratory of Natural BioResources, University of Hassiba Benbouali, Chlef, Algeria. Ali Amazouz hadjali02@gmail.com 13700319475328460025429 13700319475328460025429 No Laboratory of Natural BioResources, University of Hassiba Benbouali, Chlef, Algeria. Tahir Benyamina Douma 13700319475328460025430 13700319475328460025430 No Laboratory of Natural BioResources, University of Hassiba Benbouali, Chlef, Algeria.