Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1400
8
1
gregorian
2021
11
1
12
6
online
1
fulltext
en
Minocycline Enhance the Restorative Ability of Olfactory Ensheathing Cells by the Upregulation of BDNF and GDNF Expression After Spinal Cord Injury
Cellular and molecular Neuroscience
Cellular and molecular Neuroscience
Original
Original
<strong>Introduction:</strong> Spinal Cord Injury (SCI) is a global public health issue that results in extensive neuronal degeneration, axonal and myelin loss, and severe functional deficits. Neurotrophic factors are a potential treatment for reducing secondary damage, promoting axon growth; they are responsible for inducing myelination after injury. Olfactory Ensheathing Cells (OECs) and minocycline have promoted locomotor function after SCI. The present study investigated the neuroprotective effects of combined treatment with minocycline and OECs on spinal cord injury related to Brain-Derived Neurotrophic Factor (BDNF) and Glial Derived Neurotrophic Factor (GDNF) expressions after SCI.<br>
<strong>Methods:</strong> Adult female rats were used to experimental SCI by weight compression method. Rats received an intraperitoneal minocycline injection (90 mg/kg) immediately after SCI and 24 h after injury. OECs were transplanted one week after the injury. The hindlimb function was assessed using Basso Beattie Bresnahan (BBB) locomotor rating scale and Electromyography (EMG). After 5 weeks, the spinal cord segment centered at the injury site was removed for histopathological analysis. Immunohistological and western blot assays were performed to observe the expression of NeuN, BDNF, GDNF, and Myelin Basic Protein (MBP).<br>
<strong>Results:</strong> SCI induced the loss of locomotor function with decreased BDNF and GDNF expressions in the injury site. Minocycline+OECs increased the score of the BBB locomotor scale and increased spared tissue in the injury site. Immunohistochemical results suggested that NeuN expression significantly increased in the minocycline+OECs group than other groups. Moreover, electromyography amplitude in treated rats was increased compared to the control group. BDNF, GDNF, and MBP expressions and the number of ventral motor neurons increased further by minocycline+OECs in SCI rats. <br>
<strong>Conclusion:</strong> The present study provides evidence that minocycline may facilitate recovery of locomotor function by OECs by increasing BDNF and GDNF expressions following SCI.
Olfactory ensheathing cells, Minocycline, Spinal cord injury, Myelin basic protein, Brain-derived neurotrophic factor, Glial derived neurotrophic factor, Electromyography
777
788
http://bcn.iums.ac.ir/browse.php?a_code=A-10-1727-1&slc_lang=en&sid=1
Soheila
Pourkhodadad
s.porkhodadad@yahoo.com
13700319475328460039305
13700319475328460039305
Yes
Department of Animal Physiology, Faculty of Biology, Kharazmi University, Tehran, Iran.
Shahrbanoo
Oryan
shahrbanoo_o@yahoo.com
13700319475328460039306
13700319475328460039306
No
Department of Animal Physiology, Faculty of Biology, Kharazmi University, Tehran, Iran.
Mohammad Mehdi
Hadipour
mm_ hadipour_yahoo.com
13700319475328460039307
13700319475328460039307
No
Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Gholamreza
Kaka
gh_kaka@yahoo.com
13700319475328460039308
13700319475328460039308
No
Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Seyed Homayoon
Sadraie
sh_ sadraei@yahoo.com
13700319475328460039309
13700319475328460039309
No
Department of Anatomy, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.