Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1399
2
1
gregorian
2020
5
1
11
3
online
1
fulltext
en
Low-frequency Stimulation Decreases Hyperexcitability Through Adenosine A1 Receptors in the Hippocampus of Kindled Rats
Cellular and molecular Neuroscience
Cellular and molecular Neuroscience
Original
Original
<strong>Introduction</strong>: In this study, the role of A1 adenosine receptors in improving the effect of Low-Frequency Electrical Stimulation (LFS) on seizure-induced hyperexcitability of hippocampal CA1 pyramidal neurons was investigated.<br>
<strong>Methods</strong>: A semi-rapid hippocampal kindling model was used to induce seizures in male Wistar rats. Examination of the electrophysiological properties of CA1 pyramidal neurons of the hippocampus using whole-cell patch-clamp recording 48 h after the last kindling stimulation revealed that the application of LFS as two packages of stimulations at a time interval of 6 h for two consecutive days could significantly restore the excitability CA1 pyramidal neurons evidenced by a decreased in the of the number of evoked action potentials and enhancement of amplitude, maximum rise slope and decay slope of the first evoked action potential, rheobase, utilization time, adaptation index, first-spike latency, and post-AHP amplitude. Selective locked of A1 receptors by the administration of 8-Cyclopentyl-1,3-dimethylxanthine (1 μM, 1 μl, i.c.v.) before applying each LFS package, significantly reduced LFS effectiveness in recovering these parameters. <br>
<strong>Results</strong>: On the other hand, selective activation of A1 receptors by an injection of N6-cyclohexyladenosine (10 μM, 1 μl, i.c.v.), instead of LFS application, could imitate LFS function in improving these parameters. <br>
<strong>Conclusion</strong>: It is suggested that LFS exerts its efficacy on reducing the neuronal excitability, partially by activating the adenosine system and activating its A1 receptors.
Seizure, Kindling, Low-frequency stimulation, Electrophysiological Properties, Adenosine A1 Receptors
333
348
http://bcn.iums.ac.ir/browse.php?a_code=A-10-1713-1&slc_lang=en&sid=1
Amir
Shojaee
amshoja@gmail.com
13700319475328460027404
13700319475328460027404
No
Department of Physiology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran.
Parvin
Zareian
zareianj2011@yahoo.com
13700319475328460027405
13700319475328460027405
Yes
Department of Physiology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran.
Javad
Mirnajafi-Zadeh
mirnajaf@modares.ac.ir
13700319475328460027406
13700319475328460027406
No
Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran