Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1399
8
1
gregorian
2020
11
1
11
6
online
1
fulltext
en
Differential Dynamics of the Levels of Low Molecular Weight DNA Fragments in the Plasma of Patients With Ischemic and Hemorrhagic Strokes
Cellular and molecular Neuroscience
Cellular and molecular Neuroscience
Original
Original
<div style="text-align: justify;"><strong>Introduction: </strong>To evaluate Low-Molecular-Weight (LMW) DNA as a possible prognostic biomarker in acute ischemic and hemorrhagic stroke.<br>
<strong>Methods:</strong> LMW DNA samples were isolated from plasma and cerebrospinal fluid by phenol deproteinization, analyzed by gradient polyacrylamide electrophoresis and quantified by spectrophotometry.<br>
<strong>Results: </strong>Two common types of stroke, i.e. ischemic and hemorrhagic, differ by the temporal dynamics of cell-free DNA (cfDNA) accumulation. In hemorrhagic stroke, an initial increase in LMW DNA levels, most likely reflects an extent of the tissue damage, while in ischemic patients, the LMW DNA levels increase in parallel with the damage caused by hypoxia and subsequent compensatory reperfusion.<br>
<strong>Conclusion:</strong> These time-course data specify optimal assessment windows with maximum differentiating power for stroke outcomes: 24-48 hours post-event for ischemic stroke, and as close as possible to the moment of hospital admission for hemorrhagic stroke. These data also indicate the role of apoptosis in the formation of ischemic focus.</div>
Acute cerebrovascular accident, Cerebral ischemia, Hemorrhagic stroke, Cell-free DNA, Apoptosis
805
810
http://bcn.iums.ac.ir/browse.php?a_code=A-10-1639-1&slc_lang=en&sid=1
Irina
Vasilyeva
iravasilyeva@hotmail.com
13700319475328460030704
13700319475328460030704
Yes
Laboratory of Cancer Chemoprevention and Oncopharmacology, N.N.Petrov National Medical Research Center of Oncology, St.-Petersburg, Russia.
Vladimir
Bespalov
bespalov_niio@mail.ru
13700319475328460030705
13700319475328460030705
No
Laboratory of Cancer Chemoprevention and Oncopharmacology, N.N.Petrov National Medical Research Center of Oncology, St.-Petersburg, Russia.
Ancha
Baranova
aancha@gmail.com
13700319475328460030706
13700319475328460030706
No
Department of George, School of Systemic Biology, George Mason University, Fairfax, VA, USA.
Igor
Voznyuk
voznjouk@yandex.ru
13700319475328460030707
13700319475328460030707
No
Department of Acute Cerebrovascular Pathology and Emergency Neurology, Saint-Petersburg I.I. Dzhanelidze Research Institute for Emergency Medicine, St.-Petersburg, Russia.
Denis
Baranenko
denis.baranenko@niuitmo.ru
13700319475328460030708
13700319475328460030708
No
Department of Acute Cerebrovascular Pathology and Emergency Neurology, Saint-Petersburg I.I. Dzhanelidze Research Institute for Emergency Medicine, St.-Petersburg, Russia.