en Behavioral Neuroscience Behavioral Neuroscience Original Original <strong>Introduction</strong>: Down syndrome as a genetic disorder is a popular research topic in molecular studies. One way to study Down syndrome is via bioinformatics.&nbsp;<br> <strong>Methods</strong>: In this study, a gene expression profile from a microarray study was selected for more investigation.<br> <strong>Results</strong>: The study of Down syndrome patients shows specific genes with differential expression and network centrality properties. These genes are introduced as RHOA, FGF2, FYN, and CD44, and their level of expression is high in these patients.&nbsp;<br> <strong>Conclusion</strong>: This study suggests that besides chromosomes 21, there are additional contributing chromosomes to the risk of Down syndrome development. Besides, these genes could be used for clinical studies after more analysis. Down Syndrome, Protein interaction Maps, Transcriptome, Differentially Expressed Genes, Biological Process 79 88 http://bcn.iums.ac.ir/browse.php?a_code=A-10-941-6&slc_lang=en&sid=1 Mona Zamanian Azodi mona.azodi@gmail.com 13700319475328460031872 13700319475328460031872 No Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Mostafa Rezaei Tavirani tavirany@yahoo.com 13700319475328460031873 13700319475328460031873 Yes Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Majid Rezaei Tavirani tavirani_m@yahoo.com 13700319475328460031874 13700319475328460031874 No Department of Surgery, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Mohammad Rostami Nejad m.rostamii@gmail.com 13700319475328460031875 13700319475328460031875 No Research Institute For Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.