en Behavioral Neuroscience Behavioral Neuroscience Original Original <strong>Introduction</strong>: Sleep deprivation can cause hyperalgesia and interfere with analgesic treatments.&nbsp;The aim of the present study was to establish an obligatory sleep-abstinence model and also evaluate the effects of intracerebroventricular (ICV) injection of crocin on pain perception in Wistar rats.<br> <strong>Methods</strong>: In this experimental study, 35 adult male Wistar rats were randomly divided into 5 groups (n=7). The&nbsp;intra-ventricular cannulation was done for all rats before sleep deprivation. Sleep deprivation was performed by placing animals on a chamber equipped with an automatic animated conveyor (5 s with an interval of 3 min) for 72 h. Subsequently, the sleep-deprived animals received ICV injection of saline (MOD), Morphine 10 &micro;g (MOR), Crocin 10 ug (Cr10), and Crocin40 &micro;g (Cr40) using a microsyringe. Besides, a non-sleep-deprived group was allocated as a Control Group (NC) and only received an ICV injection of saline. Fifteen minutes after the ICV injections, pain perception was evaluated by the hot plate test (54&plusmn;0.4◦C).&nbsp;<br> <strong>Results</strong>: Compared with the NC group, latency significantly decreased in the MOD group (6.28&plusmn;0.48 vs. 4.28&plusmn; 0.48, p<0.0001). In comparison with the MOD group, both morphine (8.42&plusmn;1.53) and crocin (7.60&plusmn;1.45 for Cr10 and 8.14&plusmn;0.89 for Cr40) could significantly increase latency in the sleep-deprived animals (p<0.0001). There was no statistically significant difference between the Cr10 and Cr40 (p=0.42), Cr10, and MOR (p=0.059) and Cr40 with MOR (p=0.86) groups.<br> <strong>Conclusion</strong>: Our results indicated that crocin could attenuate hyperalgesia induced by sleep deprivation in rats.&nbsp; Secondary insomnia, Pain, Crocin, Analgesic 261 268 http://bcn.iums.ac.ir/browse.php?a_code=A-10-144-3&slc_lang=en&sid=1 Faezeh Rezaei f.rezaei2088@gmail.com 13700319475328460027365 13700319475328460027365 No Department of Anatomy, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran. Mohammad Reza Saebipour saebipour@gmail.com 13700319475328460027366 13700319475328460027366 Yes Department of Anatomy, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran. Kazem Ghaemi kazemghaemi@yahoo.com 13700319475328460027367 13700319475328460027367 No Department of Neurosurgery, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran. Mohammad Mehdi Hassanzadeh-Taheri mmhtahery35@gmail.com 13700319475328460027368 13700319475328460027368 No Department of Anatomy, Cellular and Molecular Research Center, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran. Mohsen Foadoddini foadmohsen@yahoo.com 13700319475328460027369 13700319475328460027369 No Department of Physiology, Cardiovascular Diseases Research Center, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran. Mehran Hosseini mehranhosseiny@yahoo.co.in 13700319475328460027370 13700319475328460027370 No Department of Anatomy, Cellular and Molecular Research Center, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.