Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1397
10
1
gregorian
2019
1
1
10
1
online
1
fulltext
en
Bone Marrow Stromal Cells With Exercise and Thyroid Hormone Effect on Post-Stroke Injuries in Middle-aged Mice
Cellular and molecular Neuroscience
Cellular and molecular Neuroscience
Original
Original
<div style="text-align: justify;"><strong>Introduction:</strong> Based on our previous findings, the treatment of stem cells alone or in combination with thyroid hormone (T3) and mild exercise could effectively reduce the risk of stroke damage in young mice. However, it is unclear whether this treatment is effective in aged or middle-aged mice. Therefore, this study designed to assess whether combination of Bone Marrow Stromal Cells (BMSCs) with T3 and mild treadmill exercise can decrease stroke complications in middle-aged mice. <br>
<strong>Methods:</strong> Under laser Doppler flowmetry monitoring, transient focal cerebral ischemia was produced by right Middle Cerebral Artery Occlusion (MCAO) for 45 min followed by 7 days of reperfusion in middle-aged mice. BMSCs (1×105) were injected into the right cerebral ventricle 24 h after MCAO, followed by daily injection of triiodothyronine (T3) (20 µg/100 g/d SC) and 6 days of running on a treadmill. Infarct size, neurological function, apoptotic cells and expression levels of Glial Fibrillary Acidic Protein (GFAP) were evaluated 1 week after stroke.<br>
<strong>Results:</strong> Post-ischemic treatment with BMSCs or with T3 and or mild treadmill exercise alone or in combination did not significantly change neurological function, infarct size, and apoptotic cells 7 days after ischemia in middle-aged mice (P>0.05). However, the expression of GFAP significantly reduced after treatment with BMSCs and or T3 (P<0.01). <br>
<strong>Conclusion:</strong> Our findings indicate that post-stroke treatment BMSCs with exercise and thyroid hormone cannot reverse neuronal damage 7 days after ischemia in middle-aged mice. These findings further support that age is an important variable in stroke treatment</div>
Cerebral ischemia, Combination, Bone marrow stromal cells, Thyroid hormone, Exercise, Apoptosis, Glial fibrillary acidic protein, Middle-aged, Mice
73
84
http://bcn.iums.ac.ir/browse.php?a_code=A-10-1293-1&slc_lang=en&sid=1
Kobra
Akhoundzadeh
mehr2257@gmail.com
13700319475328460019576
13700319475328460019576
No
Physiology Research Center, Semnan University of Medical Sciences, Semnan, Iran.
Abedin
Vakili
Ab.vakili@yahoo.com
13700319475328460019577
13700319475328460019577
Yes
Physiology Research Center, Semnan University of Medical Sciences, Semnan, Iran.
Hamid Reza
Sameni
hrsameni@gmail.com
13700319475328460019578
13700319475328460019578
No
Nervous System Stems Cells Research Center, Department of Anatomical Sciences, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran