Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1397
6
1
gregorian
2018
9
1
9
5
online
1
fulltext
en
Chemical Composition and Role of Opioidergic System in Antinociceptive Effect of Ziziphora Clinopodioides Essential Oil
Behavioral Neuroscience
Behavioral Neuroscience
Original
Original
<div style="text-align: justify;"><strong>Introduction:</strong> Ziziphora Clinopodioides has been used widely for various therapeutic purposes in Iranian folk medicine. The current study aimed to determine interaction of antinociceptive effect of the Essential Oil of Ziziphora Clinopodioides (EOZC) and opioidergic system in male rats using formalin test. <br>
<strong>Methods:</strong> Sixty-four male Wistar rats were divided into eight groups. The groups 1 to 7 were injected with normal saline, vehicle (Tween-80, 0.5%), 10, 20, 40 mg/kg of the EOZC, morphine (5 mg/kg) and naloxone (2 mg/kg), respectively. Thirty minutes later, the formalin test was performed by intraplantar injection of formalin (50 µL, 2%). In group 8, naloxone (2 mg/kg) was injected 15 min before injection of EOZC (20 mg/kg), followed by formalin at 15 min later. The formalin test was done as time spent for licking and biting of the injected paw. Formalin induced a biphasic pain reaction. The chemical composition of EOZC was identified using Gas Chromatography-Mass Spectrometry (GC-MS).<br>
<strong>Results:</strong> EOZC (10, 20, and 40 mg/kg) dose dependently and morphine (5 mg/kg) reduced pain responses in the both phases of pain (P<0.05). Naloxone (2 mg/kg) alone had no effect on the severity of pain (P>0.05) but pretreatment with naloxone inhibited EOZC-induced antinociception activity (P<0.05). Based on the GC-MS results, EOZC comprised 65.22% carvacrol, 19.51% thymol, 4.86% p-cymene and 4.63% γ-terpinene. <br>
<strong>Conclusion:</strong> These results demonstrate that EOZC has antinociceptive effect and this effect might mediate via opioidergic pathways.</div>
Ziziphora Clinopodioides, Antinociceptive, Opioidergic, Rat
357
366
http://bcn.iums.ac.ir/browse.php?a_code=A-10-1256-1&slc_lang=en&sid=1
Faezeh
Mohammadifard
F.mohammadiii@yahoo.com
13700319475328460017577
13700319475328460017577
No
Department of Basic Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran.
Samad
Alimohammadi
S.alimohammadi@razi.ac.ir
13700319475328460017578
13700319475328460017578
Yes
Department of Basic Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran.