en Clinical Neuroscience Clinical Neuroscience Original Original <div style="text-align: justify;"><strong>Introduction: </strong>Based on the literature, unihemispheric concurrent dual-site anodal transcranial Direct Current Stimulation (a-tDCSUHCDS) of primary Motor cortex (M1) and Dorsolateral Prefrontal Cortex (DLPFC) would be more efficient than conventional a-tDCS of M1 to induce larger and longer-lasting M1 corticospinal excitability. The main objective of the present study was to compare the effects of a-tDCSUHCDS and conventional M1 a-tDCS on the extent and durability of the motor sequence acquisition in healthy individuals.<br> <strong>Methods: </strong>In this randomized sham-controlled study, healthy volunteers were randomly divided into three groups: experimental (a-tDCSUHCDS), control (M1 a-tDCS), and sham stimulation groups. The participants practiced serial response time task over three consecutive days when they simultaneously received a-tDCS. Using the skill measure, we assessed motor learning up to 4 weeks after the completion of experimental conditions.&nbsp;<br> <strong>Results:</strong> Data analysis revealed that all groups exhibited the improved trend over the training course (P<0.001). There were no significant differences in skill acquisition among groups at post-intervention (P>0.05), while a significant improvement was observed between experimental and sham group at the retention time (P<0.05). Moreover, there were no significant differences between the control and two other groups with regard to the retention time (P>0.05).&nbsp;<br> <strong>Conclusion:</strong> These results revealed a significant increase in the skill acquisition by a-tDCSUHCDS technique with regard to retention issue, which could be a valuable finding in neuro-rehabilitation field.&nbsp;</div> Transcranial direct current stimulation, Primary motor cortex, Dorsolateral prefrontal cortex, Learning, Motor skills 59 72 http://bcn.iums.ac.ir/browse.php?a_code=A-10-1244-2&slc_lang=en&sid=1 Ailin Talimkhani ai.talimkhani@gmail.com 13700319475328460019956 13700319475328460019956 No Department of Physical Therapy, School of Rehabilitation Sciences, Hamadan University of Medical Sciences, Hamadan, Iran. Iraj Abdollahi irajabdollahi@hotmail.com 13700319475328460019957 13700319475328460019957 Yes Department of Physical Therapy, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran. Mohammad Ali Mohseni-Bandpei Mohseni_Bandpei@yahoo.com 13700319475328460019958 13700319475328460019958 No Pediatric Neurorehabilitation Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran. Fatemeh Ehsani fatemehehsani59@yahoo.com 13700319475328460019959 13700319475328460019959 No Neuromuscular Rehabilitation Research Center, Semnan University of Medical Sciences, Semnan, Iran. Sanaz Khalili Sanazkhalili94@gmail.com 13700319475328460019960 13700319475328460019960 No Department of Biostatistics and Epidemiology, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran. Shapour Jaberzadeh Shapour.jaberzadeh@monash.edu 13700319475328460019961 13700319475328460019961 No Non-invasive Brain Stimulation & Neuroplasticity Laboratory, Department of Physiotherapy, School of Primary and Allied Health Care, Faculty of Medicine, Nursing and Health Sciences, Monash University, Melbourne, Australia.