1. Introduction
Alzheimer disease (AD) is a progressive neurodegenerative disorder and the most common cause of dementia, which imposes immense suffering on patients and their families (Du, Wang & Geng, 2018). The extracellular accumulation of amyloid beta (Aβ) peptides in the cerebral cortex and the hippocampus are the pathological feature of AD, which indicates that abnormal Aβ processing plays an important role in the progression of the disease. Another feature of this disease is the reduced cholinergic neurons in the basal forebrain (Castaneda et al., 2015).
Basal forebrain cholinergic cell groups in rodents are known as the Nucleus Basalis Magnocellularis (NBM), whose branches extend to the dorsolateral surfaces of the cortical mantle, prefrontal cortex, and basolateral complex of the amygdala (Knox, 2016; Nyakas et al., 2011). It has been shown that NBM lesions impair cognition (Llorente-Ovejero et al., 2017), lower spatial learning (Hoveizi et al., 2018), and reduce Choline Acetyltransferase (ChAT) in the frontal cortex (Panocka et al., 1995). Also, ibotenic acid-induced NBM lesions decrease basal synaptic responsiveness, paired-pulse responses, and long-term potentiation (LTP) in the dentate gyrus (DG) of rats. It indicates that although NBM does not send direct cholinergic projections to the hippocampus, its lesion affects both short-term and long-term neural plasticity in the dentate gyrus (Hosseini et al., 2017). As degeneration of cholinergic neurons in the human forebrain is one of the neuropathological features of AD, animals with lesions of NBM are considered models of AD-type amnesia (Ahmed et al., 2004).
Memantine (MEM) is a noncompetitive N-methyl-D-aspartate receptor (NMDAR) antagonist clinically used to treat AD in mild to severe conditions. The effect of memantine on NMDA extra-synaptic receptors is greater than that of the NMDA synaptic receptors in the same neuron, and extrasynaptic NMDARs are largely involved in causing excitotoxicity through NMDARs in AD (Carvajal et al., 2016). Previous reports support that memantine can suppress the pathological background “noise” at therapeutically relevant concentrations but preserve or even enhance the desired physiological synaptic NMDA receptor-mediated plasticity “signal” (Parsons et al., 2007). It has been shown that appropriate concentrations of memantine alter the balance between excitation and inhibition away from inhibition in the prefrontal cortex of the neuronal circuits (Povysheva & Johnson, 2016).
Therefore, given the role of memantine in increasing the signal-to-noise ratio and its effects on the change in the balance between excitation and inhibition away from inhibition in the prefrontal cortex, also due to the effects of memantine on neural protection, there are reports regarding the amount of memantine that does not significantly change the memory of intact mice. Therefore, this study examines the effects of memantine on the spontaneous firing frequency of CA1 pyramidal neurons by using the single-unit recording technique in the Alzheimer model and intact rats.
2. Materials and Methods
Study animals
A total number of 101 male Wistar rats (200±20 g) were obtained from the Animal House of the Ahvaz Jundishapur University of Medical Sciences (AJUMS). All rats were housed at a room temperature of 20°C to 24°C under a light/darkness cycle of 12/12 h (lights on from 7:00 AM to 7:00 PM). All rats were provided with free access to food and water. Rats were divided into two groups:
Group I (n=53) includes the following subgroups:
1) Lesion+0.2 mL saline: The group had surgery, and bilateral lesions were induced in NBM by an electrical method.
2) Sham+0.2 mL saline: The group had surgery, and the electrodes were placed bilaterally into NBM without any currents.
3-5) Lesion+MEM 5 mg/kg, lesion+MEM 10 mg/kg, and lesion+MEM 20 mg/kg: The group had surgery and bilateral lesions of NBM were created by electrical method.
Group II (Intact, n=48) includes the following subgroups:
1) Intact+0.2 mL saline: This group did not undergo any surgery.
2-4) Intact+MEM 3 mg/kg, intact+MEM 5 mg/kg, and intact+MEM 10 mg/kg: These groups did not undergo any surgery.
Drug administration
The drug used in this study was memantine hydrochloride (Sigma-Aldrich, CAS Number 41100-52-1), dissolved in 0.9% injectable saline (Llorente-Ovejero et al., 2017). For each experiment, the freshly prepared solution was injected Intraperitoneally (IP).
Stereotaxic surgery
Rats were anesthetized with an intraperitoneal injection of 78 mg/kg of 10% ketamine and 3 mg/kg of 2% xylazine and placed in stereotaxic apparatus (Stoelting, USA). The head of each animal was mounted in a stereotaxic frame, and the skull was opened above NBM (AP=-1.3 mm; ML=±2.8 mm; DV=-8 mm). Then by using the Lesion Making Device (UGO Basile), the NBM electrical lesion was created in both brain hemispheres (0.5 mA for 3 s), and the Alzheimer model was created (Hoveizi et al., 2018). One week after surgery, the animals were prepared for in vivo single-unit recording.
Animal preparation for single-unit recording
Rats were deeply anesthetized with urethane (1.2-1.5 g/kg, IP; Sigma–Aldrich) (Shahidi et al., 2017). As urethane causes difficulty in breathing by increasing the liquid release to the airways and reducing the noise caused by muscle activity during breathing, the rats were subject to surgical tracheostomy before single-unit recording. Briefly, an incision was made in the midline of the neck region, and then the soft tissues and muscles were cut longitudinally to expose the animal’s trachea. At this stage, using a scalpel, a horizontal incision was made in the animal’s trachea, and a 2.5-cm polyethylene tube was sent to the lower part of the trachea. The tube was fixed with a suture, and the front of the neck was also sutured. After inserting the tube into the trachea, the animal breathed through it (Shahidi et al., 2017).
Extracellular single-unit recording
Immediately after the tracheostomy, the rat was placed in stereotaxic apparatus. Using the rat brain atlas of Paxinos and Watson and the coordinates of the dorsal hippocampus CA1 region (AP=-3.5 mm; ML=-2.2 mm; DV=-2.4 mm), a tungsten micro-electrode (FHC; America) with a diameter of 125 μm and tip impedance 5 MΩ was placed in the hippocampus CA1 region (Riahi et al., 2015). The electrode was slowly lowered to reach the pyramidal cell layer of the dorsal hippocampus, where the electrical potential transferred from the microelectrode tip was examined until a neuron with a double signal-to-noise activity ratio was found. The range in which the pyramidal cells of the CA1 region are located is 0.6 to 0.8 mm, and the electrode in this range was lowered by manual Microdrive. In the CA1 region, it is possible to take a single-unit recording from each neuron with pyramidal neuron features. The pyramidal neurons of the neurons that transmit spike signals have a constant size and shape with frequencies equal to or less than 8 spike/s (Green & Arenos, 2007). In this study, eProbe (Spike; ScienceBeam, Tehran, Iran) software was used to perform electrical recording. After finding a neuron with spontaneous activity equal to or less than 8 spike/s with a signal of the same size and shape, baseline recording was performed for 15 min. Then, the recording was continued for another 105 min in saline groups with different doses of memantine after intraperitoneal injection of saline (0.2 mL) and memantine. At this stage, the data were stored as streams, and then using eProbe software with offline sorting, they were stored for each 120 min record as Peri-Stimulus time histograms (PSTHs) with a time bin of 60 s. The average frequency was analyzed for each 120 min record in pyramidal neurons in the eProbe analysis section.
Histological verifications
To confirm the tissue in the lesion group, several animals were deeply anesthetized with an intraperitoneal injection of ketamine and xylazine after 120 minutes of single-unit recording. Their brains were removed and stabilized in 10% formalin, then the sections with a thickness of 50 μm were prepared from the lesion of NBM and the electrode entrance of dorsal hippocampus CA1. These sections were stained with hematoxylin and eosin staining (H & E) and used for comparison with the atlas of Paxinos and Watson (
Figure 1).
Statistical analysis
The study results were presented as Mean±SEM, and neurons with excessive increased or decreased responses were shown as excluded neurons in the scatterplots but removed in the next steps. The Chi-square and Fisher exact-tests were used to compare the excitatory, inhibitory, and unaffected neurons between the two groups. In addition, for measuring the effect of memantine on neural firing rate, the paired samples t-test was used in the memantine group. To evaluate the cumulative effects of memantine and saline, 1-way ANOVA and Tukey test were used. Statistical tests were performed using SPSS v. 16 and P<0.05 as the significance level.
3. Results
Effects of bilateral electrical lesions of NBM on the spontaneous responses of CA1 pyramidal neurons
As already explained, the pyramidal neurons have a frequency equal to or less than 8 spikes/s (
Figure 2A). To investigate the effect of bilateral electrical lesion of NBM on the firing of pyramidal neurons by 1-way ANOVA and post-hoc Tukey test, it was shown that the mean frequency of dorsal hippocampus CA1 pyramidal neurons after saline injections in the lesion + saline (n=14) group had a significant difference with the intact+saline (n=11) and sham+saline (n=13) groups (P<0.001). Also, it was shown that there is no significant difference between the mean frequencies of pyramidal neurons in the intact+saline and sham+saline groups (
Figure 2B).
Effects of memantine intraperitoneal injection on the spontaneous responses of CA1 pyramidal neurons in Alzheimer rat model
In this study, in the lesion+saline group, out of 20 neurons in 14 rats, 14 neurons (70%) were unaffected, 4 (20%) were excited, and 2 (10%) were inhibited. In the lesion+MEM 5 mg/kg group, out of 21 neurons in 14 rats, 12 neurons (57.14%) were unaffected, 7 (33.33%) were excited and 2 (9.5%) were inhibited. In the lesion+MEM 10 mg/kg group, out of 19 neurons in 13 rats, 6 neurons (31.6%) were unaffected, 12 (63.2%) were excited and 1 (5.2%) was inhibited. In the lesion+MEM 20 mg/kg group, out of 21 neurons in 12 rats, 5 neurons (23.8%)were unaffected, 13 (61.9%) were excited, and 3 (14.3%) were inhibited (
Figure 3A and
3B).
The Chi-square and Fisher exact-tests to compare the excitatory, inhibitory, and unaffected responses among the groups showed significant differences between the lesion+saline group with lesion+MEM 10 mg/kg (P<0.05) and Lesion+MEM 20 mg/kg (P<0.01) groups.
In this study, to investigate the effect of saline and memantine on the firing rate of pyramidal neurons, a 1-way ANOVA test, and Tukey test were used. It was shown that after injection of saline and memantine, there were significant differences between average frequencies of CA1 pyramidal neurons in the lesion+saline group with lesion+MEM 10 mg/kg (P<0.01) and lesion+MEM 20 mg/kg (P<0.001) groups (
Figure 4A).
In addition, to study the effect of memantine on the neural firing rate of excited neurons in Lesion+MEM 20 mg/kg, the paired sample t-test was used. The results of this test showed that after intraperitoneal injection of memantine in the subclass of neurons with an excitatory response (n=13), the mean firing rate was significantly different from baseline firing (P<0.001) (
Figure 4B).
The results of ANOVA and Tukey tests show that the cumulative changes in firing rate (time effect of the drug × % frequency changes from baseline) in the excited neurons of lesion+MEM 10 mg/kg (P<0.001) and lesion+MEM 20 mg/kg (P<0.001) groups had a significant difference with the lesion+saline group (
Figure 5A).
Effects of memantine intraperitoneal injection on the spontaneous responses of CA1 pyramidal neurons in intact rats
In the Intact+saline group, out of 22 neurons in 11 rats, 16 neurons (72.7%) were unaffected, 3 (13.65%) were excited, and 3 (13.65%)were inhibited. In the intact+MEM 3 mg/kg group, out of 22 neurons in 12 rats, 13 neurons (59.09%) were unaffected, and 9 (40.9%) were inhibited. In the intact+MEM 5 mg/kg group, out of 22 neurons in 13 rats, 6 neurons (27.3%) were unaffected and 16 (72.7%) were inhibited. In the intact+MEM 10 mg/kg group, out of 21 neurons in 12 rats, 3 neurons (14.3%) were unaffected and 18 (85.7%) were inhibited (
Figure 6A and
6B).
The Chi-square and Fisher exact-tests were done to compare the excitatory, inhibitory, and unaffected responses among the groups. The results showed significant differences between the intact+saline group with intact+MEM 3 mg/kg (P<0.01), intact+MEM 5 mg/kg (P<0.001), and intact+MEM 10 mg/kg (P<0.001) groups.
In this study, to investigate the effect of saline and memantine on the firing rate of pyramidal neurons in intact rats, after injection of saline and memantine, we observed a significant difference between average frequencies of CA1 pyramidal neurons in the intact+saline and Intact+MEM 10 mg/kg groups (P<0.001) (
Figure 7A).
In addition, to study the effect of memantine on the neural firing rate of inhibited neurons in the intact+MEM 10 mg/kg group, the paired sample t-test was used. The results of this test showed that after intraperitoneal injection of memantine in the subclass of neurons with an inhibited response (n=18), the mean firing rate was significantly different from baseline firing (P<0.001) (
Figure 7B).
The results of ANOVA and Tukey tests show that the cumulative changes in firing rate (time effect of the drug × % frequency changes from baseline) in inhibited neurons of the intact+MEM 10 mg/kg group had a significant difference with the intact+saline group (P<0.05) (
Figure 5B).
Comparing the effect of memantine intraperitoneal injection on the spontaneous responses of peripheral Ca1 neurons in Alzheimer model and intact rats
The Chi-square and Fisher exact-tests were done to compare the excitatory, inhibitory, and unaffected responses among the groups. The results showed a significant difference between the lesion+MEM 5 mg/kg and intact+MEM 5 mg/kg groups (P<0.001). In addition, there was a significant difference between lesion+MEM 10 mg/kg and intact+MEM 10 mg/kg groups (P<0.001) (
Figure 3B and
Figure 6B).
4. Discussion
Nervous system disorders (often before memory loss) are commonly found in elderly patients with Alzheimer disease due to the accumulation of amyloid sediments in their brains, indicating a preliminary event in the pathogenesis of Alzheimer disease (Ahnaou et al., 2017). The single-unit recording is a standard method for studying the frequency of neuron firing. The results of this study indicate that bilateral lesions of NBM and Alzheimer induction reduce the frequency of neuron firing in the hippocampal CA1 pyramidal neurons compared with intact rats, which is in line with the results of previous reports. In these reports, increasing the threshold for triggering the action potential and reducing the action potential in the hippocampal CA1 pyramidal neurons (Hatch et al., 2017), as well as the reduction of the electrical activity of the hippocampal CA1 pyramidal neurons, are shown in the transgenic mouse models. Additionally, the bilateral intra-hippocampus injection of Aβ resulted in a significant reduction in the average number of spikes/bin (Farbood et al. 2017; Shabani et al., 2016) and inhibition of long-term potentiation (LTP) induction by High-Frequency Stimulation (HFS) (Shahidi et al., 2017) in granular hippocampal dentate gyrus cells. The effect of NBM lesions was also shown on the reduction of base synapse response, paired-pulse response, and LTP in the dentate gyrus. These reports indicate that although the NBM does not introduce direct cholinergic splits to the hippocampus, its lesion affects the long-term and short-term plasticity in dentate gyrus cells (Hosseini et al., 2017).
It has been shown that the binding of memantine to NMDA receptors in the nerve tissue inhibits the entry of calcium, significantly reduces the activity of neuronal synthase nitric oxide, and protects nerve cells (Liu et al., 2017; Nyakas et al., 2011). Additionally, excessive excitation of NMDA receptors can interfere with LTP and learning. For example, intraperitoneal injection of NMDA disrupted the passive avoidance learning and inhibition of mean LTP in the hippocampal CA1 region, and the reduction of these effects was caused by meantime (Zajaczkowski et al., 1997).
The study results indicate that the neuronal firing frequency in the hippocampal CA1 pyramidal neurons in Alzheimer rats increased after the intraperitoneal injection of memantine 10 mg/kg (P<0.01) and memantine 20 mg/kg (P<0.001). In accordance with these observations, Klyubin et al. (2011) reported that memantine reduces the inhibitory effects of Aβ on the induction of LTP in CA3-to-CA1 synapses (Nyakas et al., 2011). Electrophysiological evidence suggests that memantine effectively inhibits excessive flow in the extrasynaptic NMDA receptors, while the synaptic receptor’s activity remains relatively normal (Xia et al., 2010). Li et al. showed that soluble Aβ oligomers cause excessive activity of the extrasynaptic NMDA receptors and disruptions in synaptic plasticity (Li et al., 2011). Therefore, memantine can improve memory in the Alzheimer model by inhibiting the extrasynaptic NMDA receptors.
In addition, in several studies, higher levels of acetylcholine have been reported after acute dosing of NMDA receptor antagonists (Giovannini et al., 1994; Hutson & Hogg, 1996; Ihalainen et al., 2011; Shearman et al., 2006). For example, in rats with Fimbria-fornix lesions, by the acute intraperitoneal injection of memantine 5 and 10 mg/kg, the extracellular acetylcholine levels in the hippocampus increased by 167% and 250%, respectively. In this study, 10 mg/kg of memantine increased extracellular acetylcholine levels by 3.5 times greater than the baseline level in the hippocampus and neocortex (Ihalainen et al., 2011). Electrophysiological studies have shown that memantine produces excitement effects on cholinergic signaling in the hippocampus via muscarinic receptors (Drever et al., 2007).
Electrophysiological studies also indicate that memantine binds to the site of the ion channel of the NMDA receptor with a low-affinity and voltage-dependent mode so that in the case of continued excitation in pathological conditions, this binding remains despite the loose binding of magnesium ions. However, in physiological conditions where high concentrations of glutamate are produced in transient mode, memantine is isolated from the receptor, and the transfer function in the receptor remains normal (Parsons et al., 2013). Thus, in Alzheimer disease, memantine helps the better diagnosis of physiological signals by reducing the noise background caused by glutamate signaling impairment (Danysz et al., 2000).
Generally, it seems that memantine in the Alzheimer rat model enhances the spontaneous firing frequency of hippocampal CA1 pyramidal neurons by modifying neuronal damage due to oxidative stress, decreasing LTP disorder, reducing background noise, changing the balance between excitation and inhibition away from inhibition and exciting effects on cholinergic signaling.
On the other hand, given the neuroprotective effects of memantine, several experiments have been carried out to achieve the amount of memantine that does not cause memory impairment and has tolerable side effects in intact subjects (Creeley et al., 2006). In the present study, the effect of different doses of memantine on the electrical activity of CA1 pyramidal neurons in intact rats indicated the inhibitory effects of memantine at doses of 3, 5, and 10 mg/kg on the firing frequency of these neurons. The study of the mean firing frequency of CA1 pyramidal neurons showed that 10 mg/kg memantine significantly decreased the electrical activity of these neurons (P<0.001).
Studies have shown that, in the Morris water maze test, the intraperitoneal injection of 20 mg/kg of memantine in intact rats caused short-term memory impairment, while 5 mg/kg of memantine had no effect (Duda et al., 2016). On the other hand, it has been shown that cumulative doses of memantine (4, 8, and 16 mg/kg IV) reduced the firing rate in the hippocampal CA1 in intact rats (Szegedi et al., 2010). Besides, studies in the platform relocation version of the water maze show that 5 and 10 mg/kg memantine cause cognitive impairments in 6- to 12-week-old rats (Saab et al., 2011). Additionally, acute intraperitoneal injection of 20 mg/kg memantine reduces functional memory in the Allothetic Place Avoidance Alternate Task (Wesierska et al., 2013). However, in the study by Zajaczkowski et al., the intraperitoneal injection of 20 mg/kg of memantine did not affect functional memory in the radial maze (Zajaczkowski et al., 2000). Also, some studies have shown that at the therapeutic concentrations, memantine captivates one-third of NMDA receptors and that these memantine levels have neuroprotective effects while they have no significant effect on learning (More et al., 2008). Klyubin et al. (2011) demonstrated that intraperitoneal injection of memantine 10 mg/kg at 40 min prior to HFS had no significant effect on LTP at CA3-to-CA1 synapses in intact rats. However, memantine (20 mg/kg) caused complete LTP inhibition in these synapses (Nyakas et al., 2011). Also, it was shown that 5 and 20 mg/kg of memantine have no significant effect on LTP induced by HFS in the dentate gyrus of rats (Li et al., 2013). It seems that the differences between the findings of these studies are due to the method (LTP and single-unit recording) and region of electrical recording (CA3-to-CA1 synapses, CA1 pyramidal neurons, and dentate gyrus).
The positive effects of memantine on the memory of intact mice have also been shown in some studies. For example, Zoladz et al. (2006) argued that 5 and 7.5 mg/kg of memantine in the Morris water maze test improves spatial memory in intact adult male rats. Moreover, in the radial maze test, the acute intraperitoneal injection of 0.3 and 0.56 mg/kg of memantine 20 minutes prior to the acquisition reduced the error and improved memory in the memory recall test (18 hours after the last acquisition session) (Wise & Lichtman, 2007). It seems that memantine affects memory enhancement in these cases through the imbalance between GABAergic and glutamatergic functions and higher levels of extracellular acetylcholine (Drever et al., 2007; Ihalainen et al., 2011; Povysheva & Johnson, 2016; Shearman et al., 2006; Yamaguchi et al., 2013). Also, the differences in the effective amount of memantine are related to the treatment method (oral and intraperitoneal), the duration of treatment (acute and chronic), and the treatment time (before training, immediately after training, and before recalling).
Therefore, according to the results of the current study under in vivo conditions, memantine increases the spontaneous firing frequency of CA1 pyramidal neurons in rats with electrical lesions of NBM as a model of Alzheimer disease in a dose-dependent manner. Also, in intact adult male rats, it was shown that low-dose memantine, contrary to its high dose, did not decrease the electrical activity of CA1 pyramidal neurons. So, considering the memantine neuroprotective effects reported in other studies, these findings support the use of low-dose memantine in pre-treatment studies of Alzheimer disease.
Ethical Considerations
Compliance with ethical guidelines
The Ethics Committee of Shahid Chamran University of Ahvaz approved the research protocol (Code: EE/97, 24, 3061243/scu.ac.ir).
Funding
The research project was funded by Shahid Chamran University, Ahvaz.
Authors' contributions
All authors equally contributed to preparing this article.
Conflict of interest
The authors declared no conflict of interest.
Acknowledgments
The authors would like to thank Shahid Chamran University of Ahvaz, Deputy of Research for cooperation and assistance in the project.
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