1- Cellular and Molecular Research Center, Qazvin University of Medical Science, Qazvin, Iran.
2- Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran.
3- Department of Anatomy & Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
4- Department of Anatomy & Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.; Immunogenetic Research Center, Department of Anatomy & Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
5- The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran.; Department of Anatomical Sciences, School of Medical Sciences, Bushehr University of Medical Sciences, Bushehr, Iran.
6- ENT and Head & Neck Research Center and Department, Hazrat Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran.
Abstract:
Introduction: Cell therapy has been widely considered as a therapeutic approach for neurodegenerative diseases and nervous system damage. Cholinergic neurons as one of the most important neurons that play a significant role in controlling emotions, mobility, and autonomic systems. In this study, human dental pulp stem cells (hDPSCs) were differentiated into the cholinergic neurons by β-mercaptoethanol in the preinduction phase and also by the nerve growth factor (NGF) in the induction phase.
Methods: The hDPSCs were evaluated for CD73, CD31, CD34, and Oct-4. Concentration-time relationships for NGF were assessed by evaluating the viability rate of cells and the immune response to nestin, neurofilament 160, microtubule-associated protein-2, and choline acetyltransferase.
Results: The hDPSCs had a negative response to CD34 and CD31. The optimal dose for the NGF was 50 ng/mL seven days after the induction when the highest percentage of expressing markers for the cholinergic neurons (ChAT) was detected.
Conclusion: The results of this study provided a method for producing cholinergic neurons by hDPSCs, which can be used in cytotherapy for degenerative diseases of the nervous system and also spinal cord injury.
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● Nerve growth factor increased differentiation of human dental pulp stem cells into cholinergic neurons.
● Human dental pulp stem cells were differentiated into the cholinergic neurons using βME.
● The optimal dose for nerve growth factor to induce cholinergic neural differentiation was 50 ng/mL.
Plain Language Summary
Cell therapy is a therapeutic approach in neuroregenerative medicine. Cholinergic neurons have an essential role in emotions, mobility, and autonomic systems. Here, we used human dental pulp stem cells (hDPSCs) to produce cholinergic neurons using some growth factors, such as β-mercaptoethanol and nerve growth factor (NGF). We found that β-mercaptoethanol and NGF increased the differentiation of hDPSCs into cholinergic neurons. Also, the optimal dose for NGF to induce cholinergic neural differentiation was 50 ng/mL. The protocol of this study can be used in cytotherapy in degenerative diseases of the nervous system and spinal cord injury.
Type of Study:
Original |
Subject:
Cellular and molecular Neuroscience Received: 2018/10/21 | Accepted: 2019/07/13 | Published: 2019/11/1