Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1390
6
1
gregorian
2011
9
1
2
2
online
1
fulltext
en
Inhibition of Cyclooxygenase Type 1 and 2 Enzyme by Aqueous Extract of Elaeagnus Angustifolia in Mice
Original
Original
<p align="left" ><p align="justify"> <b><font color="#221e1f" size="2">Introduction: </font><font face="Times New Roman,Times New Roman" color="#221e1f" size="2">It has been shown that the extract of Elaeagnus angustifolia can </font><font face="Times New Roman,Times New Roman" color="#221e1f" size="2">inhibit inflammation and pain induced by formalin in mice and rats. The aim </font><font face="Times New Roman,Times New Roman" color="#221e1f" size="2">of the present study is to reach evaluations of possible cellular and molecular </font><font face="Times New Roman,Times New Roman" color="#221e1f" size="2">mechanisms of Elaeagnus angustifolia extract in reducing pain and inflammation </font><font face="Times New Roman,Times New Roman" color="#221e1f" size="2">through examining the extract ability for inhibition of cyclooxygenase (Cox) type 1 and 2 enzymes and corticosterone release from adrenal glands in mice. </font><b><font color="#221e1f" size="2"><p align="justify">Methods: </p></font><font face="Times New Roman,Times New Roman" color="#221e1f" size="2">Male Swiss Webster mice were evaluated through the injection of 2 μliters to the plantar part of right foot. Elaeagnus angustifolia extract was injected to the animals 30 minutes before formalin. In order to evaluate the mechanism of extract, naloxone and memantine were administered intrapretonealy 30 minutes before the extract administration. In separate groups, after injection of extract, blood samples were taken from animals and corticosterone concentrations were measured. In an in vitro study the effect of extract on the activity of cyclooxygenase type 1 and 2 was assessed. </font><b><font color="#221e1f" size="2"><p align="justify">Results: </p></font><font face="Times New Roman,Times New Roman" color="#221e1f" size="2">the research data showed the ineffectiveness of the extract on acute phase of pain induced by formalin but it completely inhibits the chronic phase. </font><font face="Times New Roman,Times New Roman" color="#221e1f" size="2">Naloxone and Memantine administration had no effect on the efficacy of extract </font><font face="Times New Roman,Times New Roman" color="#221e1f" size="2">in the chronic phase. Also the extract administration did not increase the plasma concentration of corticosterone in mice, but in vitro inhibited Cox1 and Cox 2 enzymes. </font><b><font color="#221e1f" size="2"><p align="justify">Discussion: </p></font><font face="Times New Roman,Times New Roman" color="#221e1f" size="2">These results indicate that Elaeagnus angustifolia extract probably </font><font face="Times New Roman,Times New Roman" color="#221e1f" size="2">reducesww pain and inflammation caused by formalin in mice by inhibiting </font><font face="Times New Roman,Times New Roman" color="#221e1f" size="2">cyclooxygenase type 1 and 2 enzymes. </font></b></b></b></b></p>
Elaeagnus Angustifolia;Formalin Test;Corticosterone;Cyclooxygenase;Pain;Inflammation
31
37
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