Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1396
8
1
gregorian
2017
11
1
8
6
online
1
fulltext
en
Protective Role of Hypothermia Against Heat Stress in Differentiated and Undifferentiated Human Neural Precursor Cells: A Differential Approach for the Treatment of Traumatic Brain Injury
Cellular and molecular Neuroscience
Cellular and molecular Neuroscience
Original
Original
<div style="text-align: justify;"><strong>Introduction:</strong> The present study aimed to explore protective mechanisms of hypothermia against mild cold and heat stress on highly proliferative homogeneous human Neural Precursor Cells (NPCs) derived from Subventricular Zone (SVZ) of human fetal brain. <br>
<strong>Methods:</strong> CD133+ve enriched undifferentiated and differentiated human NPCs were exposed to heat stress at 42°C. Then, Western-blot quantification was performed using Hsp-70 (70 kilodalton heat shock proteins) recombinant protein. Finally, changes in pluripotency and Hsp-70 expression were measured using immunofluorescence staining and RT-qPCR (Quantitative reverse transcription PCR) analysis, respectively. <br>
<strong>Results: </strong>Heat stress resulted in abnormal neurospheres development. The apoptosis rate was enhanced during long-term in vitro culture of neurospheres. Neurogenic differentiation reduced and showed aberrent phenotypes during heat stress. After hypothermia treatment significant improvement in neurospheres and neuronal cell morphology was observed. <br>
<strong>Conclusion:</strong> Mild-hypothermia treatment induces attenuated heat shock response against heat stress resulting in induced HSP-70 expression that significantly improves structure and function of both undifferentiated human NPCs and differentiated neurons.</div>
Hypothermia, Neurospheres development, Neuronal phenotype, HSP-70 expression
453
466
http://bcn.iums.ac.ir/browse.php?a_code=A-10-786-1&slc_lang=en&sid=1
Sandeep Kumar
Vishwakarma
13700319475328460014727
13700319475328460014727
Yes
Central Laboratory for Stem Cell Research and Translational Medicine, Centre for Liver Research and Diagnostics, Deccan College of Medical Sciences, Hyderabad, India.
Avinash
Bardia
13700319475328460014728
13700319475328460014728
No
Central Laboratory for Stem Cell Research and Translational Medicine, Centre for Liver Research and Diagnostics, Deccan College of Medical Sciences, Hyderabad, India.
Nusrath
Fathima
13700319475328460014729
13700319475328460014729
No
Department of Genetics, Faculty of Science, Osmania University, Hyderabad, India.
Lakkireddy
Chandrakala
13700319475328460014730
13700319475328460014730
No
Central Laboratory for Stem Cell Research and Translational Medicine, Centre for Liver Research and Diagnostics, Deccan College of Medical Sciences, Hyderabad, India.
Syed
Rahamathulla
13700319475328460014731
13700319475328460014731
No
Central Laboratory for Stem Cell Research and Translational Medicine, Centre for Liver Research and Diagnostics, Deccan College of Medical Sciences, Hyderabad, India.
Nagarapu
Raju
13700319475328460014732
13700319475328460014732
No
Central Laboratory for Stem Cell Research and Translational Medicine, Centre for Liver Research and Diagnostics, Deccan College of Medical Sciences, Hyderabad, India.
Gunda
Srinivas
13700319475328460014733
13700319475328460014733
No
Centre for Cellular and Molecular Biology, Hyderabad, India.
Avinash
Raj
13700319475328460014734
13700319475328460014734
No
Centre for Cellular and Molecular Biology, Hyderabad, India.
Annamaneni
Sandhya
13700319475328460014735
13700319475328460014735
No
Department of Genetics, Faculty of Science, Osmania University, Hyderabad, India.
Vishnupriya
Satti
13700319475328460014736
13700319475328460014736
No
Department of Genetics, Faculty of Science, Osmania University, Hyderabad, India.
Santosh Kumar
Tiwari
13700319475328460014737
13700319475328460014737
No
Central Laboratory for Stem Cell Research and Translational Medicine, Centre for Liver Research and Diagnostics, Deccan College of Medical Sciences, Hyderabad, India.
Syed Ameer Basha
Paspala
13700319475328460014738
13700319475328460014738
No
Central Laboratory for Stem Cell Research and Translational Medicine, Centre for Liver Research and Diagnostics, Deccan College of Medical Sciences, Hyderabad, India.
Aleem
Ahmed Khan
aleem_a_khan@rediffmail.com
13700319475328460014739
13700319475328460014739
No
Central Laboratory for Stem Cell Research and Translational Medicine, Centre for Liver Research and Diagnostics, Deccan College of Medical Sciences, Hyderabad, India.