Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1394
10
1
gregorian
2016
1
1
7
1
online
1
fulltext
en
Impaired Memory and Evidence of Histopathology in CA1 Pyramidal Neurons through Injection of Aβ1-42 Peptides into the Frontal Cortices of Rat
Clinical Neuroscience
Clinical Neuroscience
Original
Original
<p dir="ltr" style="text-align: justify"><strong>Introduction: </strong>Alzheimer’s disease (AD) is one of the most common neurodegenerative disorders, which has much benefited from animal models to find the basics of its pathophysiology. In our previous work (Haghani, Shabani, Javan, Motamedi, & Janahmadi, 2012), a non-transgenic rat model of AD was used in electrophysiological studies. However, we did not investigate the histological aspects in the mentioned study.<br>
<strong>Methods: </strong>An AD model was developed through bilateral injection of amyloid-&beta peptides (A&beta) into the frontal cortices. Behavioral and histological methods were used to assess alterations in the memory and (ultra)structures. Furthermore, melatonin has been administered to assess its efficacy on this AD model.<br>
<strong>Results:</strong> Passive avoidance showed a progressive decline in the memory following A&beta injection. Furthermore, Nissl staining showed that A&beta neurotoxicity caused shrinkage of the CA1 pyramidal neurons. Neurodegeneration was clearly evident from Fluoro-jade labeled neurons in A&beta treated rats. Moreover, higher NF-&kappaB immunoreactive CA1 pyramidal neurons were remarkably observed in A&beta treated rats. Ultrastructural analysis using electron microscopy also showed the<br>
evidence of subcellular abnormalities. Melatonin treatment in this model of AD prevented A&beta- induced increased NF-&kappaB from immunoreaction and neurodegeneration.<br>
<strong>Discussion:</strong> This study suggests that injection of A&beta into the frontal cortices results in the memory decline and histochemical disturbances in CA1 pyramidal neurons. Furthermore, melatonin can prevent several histological changes induced by A&beta.</p>
Amyloid-β, Frontal cortex, Hippocampus, Memory, Melatonin, Animal model
31
42
http://bcn.iums.ac.ir/browse.php?a_code=A-10-473-1&slc_lang=en&sid=1
Mohammad Javad
Eslamizade
1370031947532846006180
1370031947532846006180
No
Organization
Zahra
Madjd
1370031947532846006181
1370031947532846006181
No
Organization
Homa
Rasoolijazi
1370031947532846006182
1370031947532846006182
No
Organization
Fatemeh
Saffarzadeh
1370031947532846006183
1370031947532846006183
No
Organization
Zahra
Madjd
1370031947532846006184
1370031947532846006184
No
Organization
Vahid
Pirhajati
1370031947532846006185
1370031947532846006185
No
Organization
Hadi
Aligholi
1370031947532846006186
1370031947532846006186
No
Organization
Mahyar
Janahmadi
1370031947532846006187
1370031947532846006187
No
Organization
Mehdi
Mehdizadeh
mehdizadeh.m@iums.ac.ir
1370031947532846006188
1370031947532846006188
Yes
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.