Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1394
4
1
gregorian
2015
7
1
6
3
online
1
fulltext
en
Study the Effect of Endocannabinoid System on Rat Behavior in Elevated Plus-Maze
Behavioral Neuroscience
Behavioral Neuroscience
Original
Original
<p><b>Introduction:</b> Previous studies have shown that cannabinoidergic system is involved in anxiety. However, there are controversial reports in the experimental studies. The aim of this study is to evaluate the effect of pharmacological stimulation or blocking of CB1 receptors and inhibition of endocannabinoid degradation in anxiety like behavior in elevated plus-maze (EPM) test in rat. The EPM is one of the most widely used animal models of anxiety. </p>
<div><b>Methods: </b>Male Wistar rats were randomly allocated to ten groups. Different groups of animals intraperitoneally received Win-55212 (0.3, 1 and 5 mg/kg) as CB1 receptor agonist, AM- 251 (0.3, 1 and 5 mg/kg) as CB1 receptor antagonist, URB-597 (0.03, 0.1 and 0.3 mg/kg) as endocannabinoid breakdown inhibitor or saline (as control group) 30 min before submitting into EPM test. </div>
<div><b>Results: </b>The results showed that compared to the control group, Win-55212 (1 and 5 mg/kg) and URB-597 (0.1 and 0.3 mg/kg) significantly increased both of the time and percentage of entries into open arms. AM-251 (1 and 5 mg/kg) significantly decreased the time and percentage of entries into open arms in the EPM test. These substances have no effects on the total distance covered by animals and number of closed arm entries. </div>
<div><b>Discussion: </b>It is concluded that activation of cannabinoid receptor exert anxiolytic effect while blocking of cannabinoid receptor resulted in anxiety behavior. The locomotor activity was not significantly changed by cannabinoid system. It is suggested that potentiation of cannabinoid system may be therapeutic strategy for the anxiety behavior.</div>
Anxiety, Cannabinoids, URB 597, Rat
147
153
http://bcn.iums.ac.ir/browse.php?a_code=A-10-290-3&slc_lang=en&sid=1
Alireza
Komaki
alirezakomaki@gmail.com
1370031947532846009668
1370031947532846009668
Yes
Hamadan University of Medical Sciences
Nasrin
Hashemi-Firouzi
nhashemifirozi@yahoo.com
1370031947532846009669
1370031947532846009669
No
Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Shiva
Shojaie
1370031947532846009670
1370031947532846009670
No
Department of Physiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Zobin
Souri
1370031947532846009671
1370031947532846009671
No
Department of Physiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Somayeh
Heidari
1370031947532846009672
1370031947532846009672
No
Department of Physiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Siamak
Shahidi
siamakshahidi@gmail.com
1370031947532846009673
1370031947532846009673
No
Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.