Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1394
4
1
gregorian
2015
7
1
6
3
online
1
fulltext
en
Involvement of Mu Opioid Receptor Signaling in The Protective Effect of Opioid against 6-Hydroxydopamine-Induced SH-SY5Y Human Neuroblastoma Cells Apoptosis
Behavioral Neuroscience
Behavioral Neuroscience
Original
Original
<b>
Introduction:</b> The neuroprotective role of opioid morphine against 6-hydroxydopamineinduced
cell death has been demonstrated. However, the exact mechanism(s) underlying such
neuroprotection, especially the role of subtype receptors, has not yet been fully clarified. <div><b>Methods:</b> Here, we investigated the effects of different opioid agonists on 6-OHDA-induced
neurotoxicity in human neuroblastoma SH-SY5Y cell line as an in vitro model of Parkinson’s
disease. Cell damage was induced by 150 μM 6-OHDA and the cells viability was examined by
MTT assay. Intracellular calcium, reactive oxygen species and mitochondrial membrane potential
were assessed by fluorescence spectrophotometry method. Immunoblot technique was used to
evaluate cytochrome-c and activated caspase-3 as biochemical markers of apoptosis induction. </div><div><b>Results:</b> The data showed that 6-OHDA caused significant cell damage, loss of mitochondrial
membrane potential and increase in intracellular reactive oxygen species and calcium levels
as well as activated caspase-3 and cytochrome-c release. Incubation of SH-SY5Y cells with
μ-opioid agonists, morphine and DAMGO, but not with δ-opioid agonist, DADLE, elicited
protective effect and reduced biochemical markers of cell damage and death. </div><div><b>Discussion:</b> The results suggest that μ-opioid receptors signaling participate in the opioid
neuroprotective effects against 6-OHDA-induced neurotoxicity.
</div>
Morphine, DAMGO, μ-opioid receptors, 6-hydroxydopamine, Apoptosis, SH-SY5Y cells
171
178
http://bcn.iums.ac.ir/browse.php?a_code=A-10-668-1&slc_lang=en&sid=1
Shahrzad
Eftekhar-Vaghefi
1370031947532846005751
1370031947532846005751
No
Shahid Bahonar University of Kerman. Kerman, Iran
Saeed
Esmaeili Mahani
semahani@yahoo.com
1370031947532846005752
1370031947532846005752
Yes
Shahid Bahonar University of Kerman
Leila
Elyasi
1370031947532846005753
1370031947532846005753
No
Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences. Kerman, Iran
Mehdi
Abbasnejad
1370031947532846005754
1370031947532846005754
No
Shahid Bahonar University of Kerman. Kerman, Iran