Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1393
4
1
gregorian
2014
7
1
5
3
online
1
fulltext
en
Microinfusion of Bupropion Inhibits Putative GABAergic Neuronal Activity of the Ventral Tegmental Area
Microinfusion of Bupropion Inhibits Putative GABAergic Neuronal Activity of the Ventral Tegmental Area
Behavioral Neuroscience
Behavioral Neuroscience
Original
Original
<p align="justify"><strong>Introduction:</strong> The most common interpretation for the mechanisms of antidepression is the increase of the brain monoamine levels such as dopamine (DA). The increase of DA can reduce depression but it can also decrease the monoamine release because of autoreceptor inhibition. Although bupropion can decrease the dopamine release, there is evidence about stimulatory effects of chronic application of bupropion on ventral tegmental area (VTA) neurons. In this study, the intra-VTA acute microinfusion of bupropion on putative VTA non-Dopaminergic (VTA-nonDA) neuronal firing rates was evaluated by a single neuron recording technique.</p>
<p align="justify"><strong>Methods:</strong> Animals were divided into 7 groups (sham, and 6 bupropion-microinfused groups with 1, 10-1, 10-2, 10-3, 10-4, and 10-5 mol, 1 &mul/3 min, intra-VTA). A single neuron recording technique was done according to the stereotaxic coordination. After 10 min baseline recording, ACSF or bupropion was microinfused. The recording continued to recovery period in the treated groups. The prestimulus time (PST) and interspike interval (ISI) histograms were calculated for every single unit. The assessment of the drug effect was carried out by one-way analysis of variance (ANOVA) and Post-hoc test.</p>
<p align="justify"><strong>Results:</strong> 126 non-DA neurons were separated. Bupropion could inhibit 116 neurons and 11 neurons had no significant response. Maximum inhibition was 79.1% of baseline firing rate with 44.3 min duration. The inhibitory effect of bupropion was dose-dependent.</p>
<p align="justify"><strong>Discussion:</strong> The acute inhibitory effects of bupropion on VTA-nonDA neurons can explain the fast inhibitory effects of bupropion and other antidepressants on the VTA. These data can explain some side effects of antidepressants.</p>
Bupropion,Antidepressant,Ventral Tegmental Area,Rat,Putative GABAergic,non-Dopaminergic.
182
190
http://bcn.iums.ac.ir/browse.php?a_code=A-10-1-205&slc_lang=en&sid=1
Sanaz
Amirabadi
1370031947532846006351
1370031947532846006351
No
Department of Physiology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
Firouz
Ghaderi Pakdel
1370031947532846006352
1370031947532846006352
Yes
Neurophysiology Research Center, Urmia University of Medical Sciences, Urmia, Iran.
Parviz
Shahabi
1370031947532846006353
1370031947532846006353
No
Neuroscience Research Center, Department of Physiology, Tabriz University of Medical Sciences, Tabriz, Iran.
Somayyeh
Naderi
1370031947532846006354
1370031947532846006354
No
Danesh Pey Hadi Co., Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
Mostafa
Ashrafi Osalou
1370031947532846006355
1370031947532846006355
No
Danesh Pey Hadi Co., Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
Ulker
Cankurt
1370031947532846006356
1370031947532846006356
No
Ulker Cankurt