Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1393
11
1
gregorian
2015
2
1
6
1
online
1
fulltext
en
cAMP-Epac Pathway Stimulation Modulate Connexin-43 and MicroRNA-21 Expression in Glioma Cells
Cellular and molecular Neuroscience
Cellular and molecular Neuroscience
Original
Original
<b>
Introduction: </b>Malignant astrocytic gliomas are the most common and lethal brain malignancies
due to their refractory to the current therapies. Nowadays, molecular targeted therapy has
attracted great attention in treatment of glioma. Connexin 43 (Cx43) and micro ribonucleic acid-
21(miR-21) are among molecules that are involved in glioma development and progression.
These molecules showed potential to be as target molecules with regard to glioma. Some studies
have reported that cyclic adenosine monophosphate (cAMP) signaling could be effective on
Cx43 and miR-21 in tissues other than in brain. We investigate possible relationship between
β-adrenergic receptor and its newly described downstream, exchange protein directly activated
by cAMP (Epac) signaling pathway and expression of Cx43 and miR-21 in low (1321N1) and
high grade (U87MG) glioma cell lines. <div><b>Methods: </b>We treated cells with β-adrenergic agonist and Epac activator with and without adenyl
cyclase inhibitor. Cx43 and miR-21 expression were measured with real-time PCR. </div><div><b>Results: </b>Our data showed that in 1321N1 cells, β-adrenergic-Epac pathway stimulation up and
down-regulated Cx43 and miR-21 expression respectively. Whereas, in U87MG cells these
interventions had no effect on Cx43 and miR-21 expression. </div><div><b>Discussion: </b>These findings demonstrate that low grade astrocytoma cells have better response to
our pharmacological interventions.
</div>
cAMP, Epac, Cx43, miR- 21, glioma, 1321N1, U87MG, beta adrenergic receptor
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http://bcn.iums.ac.ir/browse.php?a_code=A-10-606-1&slc_lang=en&sid=1
Hossein
Mostafavi
mostafavi73@gmail.com
1370031947532846005486
1370031947532846005486
No
Department of Physiology and Pharmacology, Zanjan University of Medical Sciences
Mojtaba
Khaksarian
mojkhaksar@yahoo.com
1370031947532846005487
1370031947532846005487
No
Department of Physiology, Lorestan University of Medical Sciences
Mohammad Taghi
Joghataei
joghataei@iums.ac.ir
1370031947532846005488
1370031947532846005488
No
Department of Neuroscience, School of Advanced Technologies in Medicine, Iran University of Medical Sciences
Sadegh
Yoosefee
yoosefee@gmail.com
1370031947532846005489
1370031947532846005489
No
Department of Neuroscience, School of Advanced Technologies in Medicine, Iran University of Medical Sciences
Mahmoud Reza
Hadjighassem
mhadjighassem@tums.ac.ir
1370031947532846005490
1370031947532846005490
Yes
Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences
Maryam
Soleimannejad
1370031947532846005491
1370031947532846005491
No
Raheleh
Gholamzadeh
1370031947532846005492
1370031947532846005492
No
Sanam
Seifollahi Bahnamiri
1370031947532846005493
1370031947532846005493
No