Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1392
11
1
gregorian
2014
2
1
5
1
online
1
fulltext
en
Detection of Interleukin-19 mRNA in C57BL/6 Mice Astroglial Cells and Brain Cortex
Behavioral Neuroscience
Behavioral Neuroscience
Original
Original
<p><b>Introduction:</b> Astrocytes are the most abundant glial cell type. In addition to their neurological roles, astrocytes also have immune functions. They have been involved in antigen presentation in the central nervous system (CNS). Activated astrocytes express adhesion molecules, chemokines and release several inflammatory mediators, pro-inflammatory cytokines, neurotrophic and neuroprotective factors, thus these cells have a dual role within the CNS: neuroinflammation and repair processes. IL-19, IL-20, IL-22, IL-24, IL-26, IL-28A, IL-28B, and IL-29 are members of the IL-10 family of cytokines. These cytokines have different biological functions in spite of partial amino acid sequences homology. Signal transduction of the IL-10 family of cytokines is through R1-type and R2-type receptors. Methods: No information has been available about the expression and regulation of IL-19 in mice astrocytes and brain. To investigate the expression of IL-19, we examined its expression in C57BL/6 mice astroglial cells in response to lipopolysaccharide (LPS), using reversetranscription polymerase chain reaction (RT-PCR) method. </p>
<div><b>Results:</b> We provide for the first time, evidence that astrocytes can express IL-19 mRNA following LPS stimulation. Furthermore, we have found the expression of IL-19 mRNA in the cortex of adult C57BL/6 mice following intraperitoneal (i.p.) administration of LPS. </div>
<div>Discussion: This finding will contribute to current knowledge on the function and behavior of cells and mediators during inflammatory conditions in the brain</div>
IL-19, Mice Astroglial Cells, brain Cortex, Lipopolysaccharide.
88
95
http://bcn.iums.ac.ir/browse.php?a_code=A-10-1-200&slc_lang=en&sid=1
Bahareh
Abd Nikfarjam
1370031947532846006464
1370031947532846006464
No
Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Massoumeh
Ebtekar
1370031947532846006465
1370031947532846006465
Yes
Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Farzaneh
Sabouni
1370031947532846006466
1370031947532846006466
No
National Institutes of Genetic Engineering and Biotechnology, Tehran, Iran.
Zahra
Pourpak
1370031947532846006467
1370031947532846006467
No
Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences and Health Services, Tehran, Iran.
Maryam
Kheirandish
1370031947532846006468
1370031947532846006468
No
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.