Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1388
10
1
gregorian
2010
1
1
1
2
online
1
fulltext
en
Evaluation of nitric oxide involvement in effect of lead on dependency to morphine in mice
Original
Original
<p dir="LTR" style="text-align: justify;"><span style="font-size:12px;">In the present study, interactions between lead exposure with nitric oxide precursor (L-arginine) or nitric oxide synthase (NOS) inhibitor (L-NAME) on naloxone-induced jumping and diarrhea in morphine-dependent mice were examined. Chronic lead acetate (0.05%) exposure altered naloxone-induced jumping and diarrhea in mice. Jumping was decreased after 7 days and was unchanged 14 and 28 days after lead exposure. Diarrhea was only increased 28 days after lead exposure, which shows a difference between two signs of withdrawal syndrome. Since jumping is the most important sign, the animals were exposed to lead for 7 days in the rest of experiments. In a set of experiments, the nitric oxide agents (L-arginine) or L-NG-nitro arginine methyl ester (L-NAME) were used before naloxone injection to test their effects on the expression of jumping. The low dose of L-arginine, a precursor of nitric oxide (20 mg/kg) decreased jumping, but increased diarrhea. Higher dose of L-arginine (80 mg/kg) increased jumping, while decreased diarrhea. L-NAME decreased both jumping and diarrhea. On the other hand, L-arginine in combination with lead reversed lead-induced attenuation of naloxone-induced jumping, while decreased diarrhea. L-NANE in combination with lead decreased diarrhea, while did not alter jumping. In the second set of experiments, nitric oxide drugs were injected during development of morphine dependency. Data showed that jumping was increased or decreased by low or higher dose of L-arginine respectively. Diarrhea was also increased by the drug. L-NAME decreased both jumping and diarrhea in the development of morphine dependency. Both L-arginine and L-NAME in combination with lead decreased lead-induced jumping and diarrhea. It is concluded that nitric oxide may modulate morphine withdrawal signs and lead-induced attenuation of jumping.</span></p>
Nitric oxide, Lead, Dependency, Mice
3
9
http://bcn.iums.ac.ir/browse.php?a_code=A-10-1-18&slc_lang=en&sid=1
Mohammad-Reza
Zarrindast
1370031947532846008181
1370031947532846008181
Yes
Bita
Hamidi
1370031947532846008182
1370031947532846008182
No
Mohammad
Sharifzadeh
1370031947532846008183
1370031947532846008183
No
Mousa
Sahebgharani
1370031947532846008184
1370031947532846008184
No
Soheila
Fazli-Tabaei
1370031947532846008185
1370031947532846008185
No