Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1401
2
1
gregorian
2022
5
1
13
3
online
1
fulltext
en
Prenatal Zinc Supplementation Ameliorates Hippocampal Astrocytes Activation and Inflammatory Cytokines Expression Induced by Lipopolysaccharide in a Rat Model of Maternal Immune Activation
Cellular and molecular Neuroscience
Cellular and molecular Neuroscience
Original
Original
<div style="text-align: justify;"><strong>Introduction</strong>: Evidence suggests that gestational exposure to Lipopolysaccharide (LPS) results in fetal zinc deficiency and eventually neurodevelopmental abnormalities. In this study, we utilized a rat model of Maternal Immune Activation (MIA) to investigate the possible neuroprotective effects of zinc supplementation during pregnancy on hippocampal astrocytes activation as well as inflammatory cytokines expression in adult offspring.<br>
<strong>Methods</strong>: Pregnant rats received intraperitoneal injections of either LPS (0.5 mg/kg) or saline on Gestational Days (GD) 15 and 16, and orally gavaged with zinc sulfate (30 mg/kg) during pregnancy. Astrocyte density and histological assessment were evaluated in the hippocampus of adult offspring on Postnatal Days (PND) 60 to 62. Also, the mRNA levels of IL-6, TNF-α, IL-1β, NF-κB, and GFAP were measured using qPCR analysis.<br>
<strong>Results</strong>: Prenatal exposure to LPS resulted in upregulated expression levels of IL-6, TNF-α, NF-κB, and GFAP in the hippocampus of adult pups. Moreover, the offspring from the LPS group showed an increased astrocyte density in the CA1 region with no histological alterations in CA1 and CA3 areas. However, maternal zinc supplementation ameliorated the LPS-induced inflammatory alterations.<br>
<strong>Conclusion</strong>: This study supports the premise that zinc supplementation during pregnancy might be an early treatment option to inhibit hippocampal inflammation induced by the maternal immune response to infectious agents. </div>
Maternal immune activation, Maternal zinc supplementation, Schizophrenia, Lipopolysaccharide, Hippocampus, Inflammatory markers
335
348
http://bcn.iums.ac.ir/browse.php?a_code=A-10-3361-1&slc_lang=en&sid=1
Ebrahim
Savareh
Ebrahim.Savareh@hums.ac.ir
13700319475328460041424
13700319475328460041424
No
Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Nahid
Davoodian
Nahid_Davoodian@yahoo.com
13700319475328460041425
13700319475328460041425
Yes
Endocrinology and Metabolism Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Ronak
Mousaviyan
Ronak.Mousaviyan@hums.ac.ir
13700319475328460041426
13700319475328460041426
No
Department of Clinical Biochemistry, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Maryam
Ghasemi-Kasman
maryam.ghasemi65@gmail.com
13700319475328460041427
13700319475328460041427
No
Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Science, Babol, Iran.
Ali
Atashabparvar
Ali.Atashabparvar@hums.ac.ir
13700319475328460041428
13700319475328460041428
No
Department of Pathology, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Ebrahim
Eftekhar
Ebrahim.Eftekhar@hums.ac.ir
13700319475328460041429
13700319475328460041429
No
Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.