Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1402
8
1
gregorian
2023
11
1
14
6
online
1
fulltext
en
Effectiveness of Nicotinamide Phosphoribosyltransferase/Pre-B Cell Colony-enhancing Factor/ Visfatin in preventing High Glucose-induced Neurotoxicity in an In-vitro Model of Diabetic Neuropathy
Cellular and molecular Neuroscience
Cellular and molecular Neuroscience
Original
Original
<div style="text-align: justify;"><span style="line-height:2;"><span style="font-size:14px;"><span style="font-family:Tahoma;"><strong>Introduction</strong>: Diabetic neuropathy is a well-known complication of diabetes. Recently, hyperglycemia-induced toxicity has been confirmed to participates in multiple cellular pathways typical for neural deterioration. Nicotinamide phosphoribosyltransferase/pre-b cell colony-enhancing factor (Nampt/PBEF)/visfatin is a novel endogenous ligand that some studies have shown its neuroprotective effects on neurodegenerative disease. Therefore, we hypothesized that visfatin may prevent high glucose (HG)-induced neurotoxicity by inhibiting apoptosis, autophagy, and reactive oxygen species (ROS) responses properly.<br>
<strong>Methods</strong>: In this study, pheochromocytoma cell line 12 (PC12) cells were exposed to both HG concentrations (50, 75, 100, 125, 150 mM) and visfatin (50, 100, 150 ng/mL) at different time -points to determine the optimum time and dose of glucose and visfatin. To investigate the effects of visfatin on HG-induced damage in the PC12 diabetic neuropathy model, we examined ROS response, apoptosis, and autophagy using ROS detection kit, flow cytometry, and real-time PCR/Western blot, respectively.<br>
<strong>Results</strong>: We determined that HG concentration significantly increased the ROS level and apoptosis of diabetic PC12 cells. However, visfatin treatment significantly decreased the ROS production (P<0.05) and apoptosis of diabetic PC12 cells (P<0.0001). Beclin-1 messenger ribonucleic acid (mRNA) level (P<0.05) and light chain 3 (Lc3)-II protein level (P<0.05) showed that the autophagy pathway is impaired by HG concentrations. <br>
<strong>Conclusion</strong>: We concluded that visfatin can sufficiently decrease neural damage caused by ROS production and apoptosis under HG-induced toxicity.</span></span></span></div>
Visfatin, Diabetic neuropathy, Antioxidant, Apoptosis, Autophagy
867
878
http://bcn.iums.ac.ir/browse.php?a_code=A-10-2870-2&slc_lang=en&sid=1
Sarvin
Jahanbani
Sarvin@gmail.com
13700319475328460047593
0009-0009-5057-484X
No
Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
Mehdi
khaksari
khaksari417@yahoo.com
13700319475328460047594
13700319475328460047594
No
Addiction Research Center, Shahroud University of Medical Sciences, Shahroud, Iran.
Fatemeh Sadat
Bitaraf
Bitaraff@gmail.com
13700319475328460047595
13700319475328460047595
No
Department of Medical Biotechnology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
Majid
Rahmati
Majidrahmati@hotmail.com
13700319475328460047596
13700319475328460047596
No
Cancer Prevention Research Center, Shahroud University of Medical Sciences, Shahroud, Iran.
Kobra
Foroughi
Foroughik@gmail.com
13700319475328460047597
13700319475328460047597
No
Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
Asghar
Shayannia
a.shayannia@shmu.ac.ir
13700319475328460047598
13700319475328460047598
Yes
Department of Medical Biotechnology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.