Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1401
6
1
gregorian
2022
9
1
13
5
online
1
fulltext
en
In Vitro Assessment of the Gene Expression of EZH-2 and P300 During Motor Neuron Differentiation of Human Umbilical Cord Blood Mesenchymal Stem Cells
Cellular and molecular Neuroscience
Cellular and molecular Neuroscience
Original
Original
<span style="font-size:14px;"><span style="font-family:Tahoma;"><strong>Introduction: </strong>Maintenance of neurogenesis depends on the function of some histone-modifying enzymes; including Enhancer of zeste homolog 2 (EZH2) and histone acetyltransferases (P300). The mechanism of epigenetic regulation and gene expression underlying the transition of human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) into MNs has not been fully clarified. <br>
<strong>Methods: </strong>Two morphogens; sonic hedgehog (Shh: 100 ng/mL) and retinoic acid (RA: 0.01 mM) were involved in the specification of hUCB-MSCs into MNs after MSC characterization using Flow cytometry. Real time-quantitative PCR and immunocytochemistry were performed to find the expression of the genes at the level of mRNA and protein. <br>
<strong>Results:</strong> The expression of MN-related markers was confirmed at the level of mRNA and protein by induction of differentiation. The results were confirmed by immunocytochemistry and showed those mean cell percentages of 55.33%±15.885% and 49.67%±13.796% could express Islet-1 and ChAT, respectively. The gene expression level of Islet-1 and ChAT was significantly increased in the first and second week of exposure, respectively. After two weeks, the expression level of P300 and EZH-2 genes increased remarkably. No significant expression of Mnx-1 was detected when compared to the control sample.<br>
<strong>Conclusion:</strong> MN-related markers, Islet-1 and ChAT, were detected in differentiated cells of hUCB-MSCs, supporting the potency of cord blood cells in the regeneration of MN-related disorders. Assessing these epigenetic regulatory genes at the protein level can be suggested to confirm their functional epigenetic modifying effects during motor neuron differentiation.<br>
<span style="line-height:200%"><span style="color:#4bacc6"></span></span></span></span>
Cord blood, Motor Neuron, P300, EZH-2
709
718
http://bcn.iums.ac.ir/browse.php?a_code=A-10-2997-1&slc_lang=en&sid=1
Marjaneh
Motaghed
marjanehmd@gmail.com
13700319475328460042828
13700319475328460042828
No
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
Davood
Sanooghi
davoodsanoghi@gmail.com
13700319475328460042829
13700319475328460042829
No
Department of Genetics, School of Biological Sciences, Shahid Beheshti University, Tehran, Iran.
Zohreh
Bagher
baharebagher@gmail.com
13700319475328460042830
13700319475328460042830
No
ENT and Head and Neck Research Center and Department, Five Senses Institute, Hazrat Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran.
Faezeh
Faghihi
faezefaghihi@yahoo.com
13700319475328460042831
13700319475328460042831
Yes
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
Abolfazl
Lotfi
Lotfi.A@tvu.ac.ir
13700319475328460042832
13700319475328460042832
No
Damavand Agricultural College, Technical and Vocational University, Tehran, Iran.
Ali
Shahbazi
shahbazial@yahoo.com
13700319475328460042833
13700319475328460042833
No
Department of Neuroscience, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
Mohammad
Taghi Jogataei
joghataei.mt@iums.ac.ir
13700319475328460042834
13700319475328460042834
No
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.