Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1390
7
1
gregorian
2011
10
1
3
1
online
1
fulltext
en
The Effect of Scopolamine on Avoidance Memory and Hippocampal Neurons in Male Wistar Rats
Clinical Neuroscience
Clinical Neuroscience
Original
Original
<p align="left" ><font color="#221e1f" size="2"><font color="#221e1f" size="2"><p align="left" ><p><strong><font color="#221e1f" size="2"><font color="#221e1f" size="2">Introduction: </font></font><font color="#221e1f" face="Times New Roman,Times New Roman" size="2"><font color="#221e1f" face="Times New Roman,Times New Roman" size="2"><font color="#221e1f" face="Times New Roman,Times New Roman" size="2">Cholinergic systems are involved in learning and memory. Scopolamine, a muscarinic acetylcholine receptor antagonist, is used as a standard/ </font></font></font><font color="#221e1f" face="Times New Roman,Times New Roman" size="2"><font color="#221e1f" face="Times New Roman,Times New Roman" size="2"><font color="#221e1f" face="Times New Roman,Times New Roman" size="2">reference drug for inducing cognitive deficits in healthy humans and animals. The </font></font></font><font color="#221e1f" face="Times New Roman,Times New Roman" size="2"><font color="#221e1f" face="Times New Roman,Times New Roman" size="2"><font color="#221e1f" face="Times New Roman,Times New Roman" size="2">purpose of this study was to evaluate the effects of scopolamine on avoidance memory and number of neurons in rat’s hippocampus. . </font></font></font></strong><font color="#221e1f" size="2"><font color="#221e1f" size="2"><p align="justify"><strong>Methods: </strong></p></font></font><font color="#221e1f" face="Times New Roman,Times New Roman" size="2"><font color="#221e1f" face="Times New Roman,Times New Roman" size="2"><font color="#221e1f" face="Times New Roman,Times New Roman" size="2">Thirty five male albino Wistar rats (200 ± 20 g) were used in this study. The rats were divided randomly into five groups: control group (healthy samples), </font></font></font><font color="#221e1f" face="Times New Roman,Times New Roman" size="2"><font color="#221e1f" face="Times New Roman,Times New Roman" size="2"><font color="#221e1f" face="Times New Roman,Times New Roman" size="2">sham (saline) and 3 experimental groups 0.2, 0.5 and 1 mg/kg (intraperitoneally - single dose of Scopolamine). Animals were tested by passive avoidance method (shuttle box). After one week, a memory test was taken from rats. Finally, with dissection of the rats' brains and tissue operations, neurons were stained with cresyl violet. Photographs of the samples in hippocampal areas were prepared, and neurons were counted. </font></font></font><font color="#221e1f" size="2"><font color="#221e1f" size="2"><p align="justify"><strong>Results: </strong></p></font></font><font color="#221e1f" face="Times New Roman,Times New Roman" size="2"><font color="#221e1f" face="Times New Roman,Times New Roman" size="2"><font color="#221e1f" face="Times New Roman,Times New Roman" size="2">Our results showed that the number of neurons in all experimental groups was lower than that in the control group. The highest decrease in number of neurons was shown in response to 1 mg/kg scopolamine compared to the control group in all regions of hippocampus. Also, we found that in comparison to the saline-treated animals, the injection of scopolamine to rats after training, caused memory destruction. </font></font></font><font color="#221e1f" size="2"><font color="#221e1f" size="2"><p><strong>Discussion: </strong></p><p></p><p></p></font></font><font color="#221e1f" face="Times New Roman,Times New Roman" size="2"><font color="#221e1f" face="Times New Roman,Times New Roman" size="2"><font color="#221e1f" face="Times New Roman,Times New Roman" size="2">We concluded that memory impairment-induced by scopolamine is probably associated with neuronal loss and this decrease was dose dependent. </font></font></font></p></font></font><font color="#221e1f" face="Times New Roman,Times New Roman" size="2"><font color="#221e1f" face="Times New Roman,Times New Roman" size="2"><font color="#221e1f" face="Times New Roman,Times New Roman" size="2"><strong> </strong></font></font></font>
Scopolamine,Hippocampus,Neurons,Passive Avoidance Memory
9
15
http://bcn.iums.ac.ir/browse.php?a_code=A-10-1-82&slc_lang=en&sid=1
Saba
Seifhosseini
1370031947532846001461
1370031947532846001461
Yes
Mehrdad
Jahanshahi
1370031947532846001462
1370031947532846001462
No
Ali
Moghimi
1370031947532846001463
1370031947532846001463
No
Nasrin-Sadat
Aazami
1370031947532846001464
1370031947532846001464
No