Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1401
6
1
gregorian
2022
9
1
13
5
online
1
fulltext
en
Caffeic Acid Phenethyl Ester With Mesenchymal Stem Cells Improves Behavioral and Histopathological Changes in the Rat Model of Parkinson Disease
Cellular and molecular Neuroscience
Cellular and molecular Neuroscience
Original
Original
<div style="text-align: justify;"><strong>Introduction: </strong>Parkinson disease (PD) results from the destruction of dopaminergic neurons in the brain. This study aimed to investigate the protective effects of natural antioxidants such as caffeic acid phenethyl ester (CAPE) to maintain these neurons.<br>
<strong>Methods: </strong>CAPE is one of the main ingredients of propolis. Intranasal administration of 1-methyl-4-phenyl-2;3;4;6-tetrahydropyridine (MPTP) was used to generate a PD model in rats. A total of 2×bone marrow stem cells (BMSCs) were injected from the tail vein. Behavioral tests, immunohistochemistry, DiI, cresyl fast violet, and TUNEL staining were used to evaluate the rats 2 weeks after treatment.<br>
<strong>Results:</strong> In all treatment groups with stem cells, the DiI staining method revealed that the cells migrated to the substantia nigra pars compacta after injection. Treatment with CAPE significantly protects dopaminergic neurons from MPTP. The highest number of tyrosine hydroxylase (TH) positive neurons was seen in the pre-CAPE+PD+stem cell (administration of CAPE, then the creation of PD, finally injection of stem cells) group. The number of TH+cells in all groups that received CAPE was significant compared to groups that received the stem cells only (P<0.001). Intranasal administration of MPTP significantly increases the number of apoptotic cells. The lowest number of apoptotic cells was in the CAPE+PD+stem cell group. <br>
<strong>Conclusion:</strong> The results showed that the use of CAPE and stem cells in Parkinson rats caused a significant reduction in the apoptotic cells.<br>
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Parkinson disease, MPTP, Antioxidant, Caffeic acid phenethyl ester, TUNNEL staining
637
646
http://bcn.iums.ac.ir/browse.php?a_code=A-10-1398-1&slc_lang=en&sid=1
Khojasteh
Rahimi Jaberi
khrakhimijaberi@gmail.com
13700319475328460042982
13700319475328460042982
No
Department of Anatomy, Nervous System Stem Cell Research Center, Semnan University of Medical Sciences, Semnan, Iran.
Manouchehr
Safari
kh_safari@yahoo.com
13700319475328460042983
13700319475328460042983
Yes
Department of Anatomy, Nervous System Stem Cell Research Center, Semnan University of Medical Sciences, Semnan, Iran.
Vahid
Semnani
vahidsemnani1345@yahoo.com
13700319475328460042984
13700319475328460042984
No
Department of Pathology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
Hamid Reza
Sameni
hrsameni@gmail.com
13700319475328460042985
13700319475328460042985
No
Department of Anatomy, Nervous System Stem Cell Research Center, Semnan University of Medical Sciences, Semnan, Iran.
Sam
Zarbakhsh
szarbakhsh@yahoo.com
13700319475328460042986
13700319475328460042986
No
Department of Anatomy, Nervous System Stem Cell Research Center, Semnan University of Medical Sciences, Semnan, Iran.
Laya
Ghahari
laya_gh@yahoo.com
13700319475328460042987
13700319475328460042987
No
Department of Anatomy, School of Medicine, AJA University of Medical Sciences, Tehran, Iran.