Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1400
10
1
gregorian
2022
1
1
13
1
online
1
fulltext
en
Effect of Hydroalcoholic Extract of Stachys lavandulifolia on Pentylenetetrazole-induced Seizures in Male Mice: The Role of GABAergic and Opioidergic Systems
Behavioral Neuroscience
Behavioral Neuroscience
Original
Original
<div style="text-align: justify;"><strong>Introduction:</strong> Epilepsy is one of the most common neurological disorders. Though there are several effective drugs for treating epilepsy, most drugs are associated with side effects and drug interactions. Stachys lavandulifolia used in Iranian traditional medicine has proven anti-anxiety and sedative properties. The current study aimed to evaluate the anticonvulsant effect of hydroalcoholic extract of S. lavandulifoliaon the Pentylenetetrazole (PTZ)-induced seizure in male mice and the role of benzodiazepine and opioid receptors. <br>
<strong>Methods</strong>: This study was conducted on 100 male mice, randomly categorized into 10 groups: Normal Saline (NS), two diazepam groups (0.025 and 0.1 mg/kg), three S. lavandulifolia extract groups (50, 100, and 200 mg/kg), diazepam 0.025 mg/kg+S. lavandulifolia extract 50 mg/kg, and three groups that pretreated with NS, flumazenil, or naloxone, 5 min before injection of 200 mg/kg S. lavandulifolia extract. After 30 min, PTZ (80 mg/kg) was injected into animals, and seizure indices were evaluated. <br>
<strong>Results:</strong> The S. lavandulifoliaextract attenuated the PTZ-induced seizures in a dose-dependent manner, and pretreatment with flumazenil reversed this effect. However, pretreatment with naloxone could not reverse this effect because seizure indices in the naloxone pretreated group were lower than that in the normal saline group. The combination of an ineffective dose of diazepam and S. lavandulifoliaextract decreased PTZ-induced seizures. <br>
<strong>Conclusion:</strong> The results of our study showed the anticonvulsant properties of hydroalcoholic extract of S. lavandulifolia. These effects might be due to the impact of the components of this extract on the central benzodiazepine system. </div>
Stachys lavandulifolia, Pentylenetetrazole, Seizure, Flumazenil, Naloxone
107
116
http://bcn.iums.ac.ir/browse.php?a_code=A-10-2299-1&slc_lang=en&sid=1
Hamid
Behzadnia
hamidbehzadnia7@gmail.com
13700319475328460040468
13700319475328460040468
No
Department of Neurosurgery, Poursina Hospital, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
Amin
Naseri
dr.amin_ms84@yahoo.com
13700319475328460040469
13700319475328460040469
No
Department of Neurosurgery, Poursina Hospital, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
Mohammadreza
Emamhadi
emamhadi42@yahoo.com
13700319475328460040470
13700319475328460040470
No
Brachial Plexus and Peripheral Nerve Injury Center, Department of Neurosurgery, Poursina Hospital, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
Shervin
Ghadarjani
shervin.gh@gmail.com
13700319475328460040471
13700319475328460040471
No
Department of Neurosurgery, Poursina Hospital, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
Iraj
Aghaei
aghaei@gums.ac.ir
13700319475328460040472
13700319475328460040472
Yes
Neuroscience Research Center, Poursina Hospital, Guilan University of Medical Sciences, Rasht, Iran.
Ahmadreza
Dehpour
dehpour@yahoo.com
13700319475328460040473
13700319475328460040473
No
Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran.