Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1398
6
1
gregorian
2019
9
1
10
5
online
1
fulltext
en
Protective Effects of Nucleobinding-2 After Cerebral Ischemia Via Modulating Bcl-2/Bax Ratio and Reducing Glial Fibrillary Acid Protein Expression
Behavioral Neuroscience
Behavioral Neuroscience
Original
Original
<div style="text-align: justify;"><strong>Introduction</strong>: Nucleobinding-2 (NUCB2) or nesfatin-1, a newly identified anorexigenic peptide, has antioxidant, anti-inflammatory, and anti-apoptotic properties. Brain ischemia-reperfusion induces irreversible damages, especially in the hippocampus area. However, the therapeutic effects of NUCB2 have not been well investigated in cerebral ischemia. This study was designed for the first time to investigate the protective effects of NUCB2/Nesfatin-1 on the expression of apoptosis-related proteins and reactive astrogliosis level in the CA1 area of hippocampus in an experimental model of transient global cerebral ischemia.<br>
<strong>Methods</strong>: The male Wistar rats were randomly allocated into 4 groups (sham, NUCB2, ischemia-reperfusion, and ischemia-reperfusion+NUCB21) (n =7). The model of cerebral ischemia was prepared by common carotid arteries occlusion for 20 minutes. Nesfatin-1 (20 µg/kg) and saline (as a vehicle) were injected (intraperitoneally) at the beginning of the reperfusion period. The assessment of the protein expression levels was performed by immunofluorescence and immunohistochemical staining.<br>
<strong>Results</strong>: NUCB2 significantly reduced the Bax and GFAP protein levels in the CA1 area after ischemia (P<0.05). Also, NUCB2 increased Bcl-2 protein level (P<0.05). NUCB2 exerted protective effects against ischemic injury by the inhibition of astrocytes activation as an inflammatory response and decreased neuronal cell apoptosis.<br>
<strong>Conclusion</strong>: The present study provides the possible neuroprotective view of nesfatin-1 in the treatment of ischemia injury model in rat hippocampus.</div>
Nucleobinding-2 (NUCB2), Nesfatin-1, Apoptosis, Astrogliosis, Hippocampus, Ischemia
451
460
http://bcn.iums.ac.ir/browse.php?a_code=A-10-135-3&slc_lang=en&sid=1
Soheila
Erfani
sohailaerfani@gmail.com
13700319475328460024305
13700319475328460024305
No
Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
Ali
Moghimi
moghimi@um.ac.ir
13700319475328460024306
13700319475328460024306
No
Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
Nahid
Aboutaleb
dr.nabotaleb@gmail.com
13700319475328460024307
13700319475328460024307
Yes
Department of Physiology, Faculty of Medicine, Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran.
Mehdi
Khaksari
khaksari417@yahoo.com
13700319475328460024308
13700319475328460024308
No
Addiction Research Center, Shahroud University of Medical Sciences, Shahroud, Iran.