Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1399
2
1
gregorian
2020
5
1
11
3
online
1
fulltext
en
Intra-cerebroventricular Administration of Crocin Attenuates Sleep Deprivation-induced Hyperalgesia in Rats
Behavioral Neuroscience
Behavioral Neuroscience
Original
Original
<strong>Introduction</strong>: Sleep deprivation can cause hyperalgesia and interfere with analgesic treatments. The aim of the present study was to establish an obligatory sleep-abstinence model and also evaluate the effects of intracerebroventricular (ICV) injection of crocin on pain perception in Wistar rats.<br>
<strong>Methods</strong>: In this experimental study, 35 adult male Wistar rats were randomly divided into 5 groups (n=7). The intra-ventricular cannulation was done for all rats before sleep deprivation. Sleep deprivation was performed by placing animals on a chamber equipped with an automatic animated conveyor (5 s with an interval of 3 min) for 72 h. Subsequently, the sleep-deprived animals received ICV injection of saline (MOD), Morphine 10 µg (MOR), Crocin 10 ug (Cr10), and Crocin40 µg (Cr40) using a microsyringe. Besides, a non-sleep-deprived group was allocated as a Control Group (NC) and only received an ICV injection of saline. Fifteen minutes after the ICV injections, pain perception was evaluated by the hot plate test (54±0.4◦C). <br>
<strong>Results</strong>: Compared with the NC group, latency significantly decreased in the MOD group (6.28±0.48 vs. 4.28± 0.48, p<0.0001). In comparison with the MOD group, both morphine (8.42±1.53) and crocin (7.60±1.45 for Cr10 and 8.14±0.89 for Cr40) could significantly increase latency in the sleep-deprived animals (p<0.0001). There was no statistically significant difference between the Cr10 and Cr40 (p=0.42), Cr10, and MOR (p=0.059) and Cr40 with MOR (p=0.86) groups.<br>
<strong>Conclusion</strong>: Our results indicated that crocin could attenuate hyperalgesia induced by sleep deprivation in rats.
Secondary insomnia, Pain, Crocin, Analgesic
261
268
http://bcn.iums.ac.ir/browse.php?a_code=A-10-144-3&slc_lang=en&sid=1
Faezeh
Rezaei
f.rezaei2088@gmail.com
13700319475328460027365
13700319475328460027365
No
Department of Anatomy, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
Mohammad Reza
Saebipour
saebipour@gmail.com
13700319475328460027366
13700319475328460027366
Yes
Department of Anatomy, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
Kazem
Ghaemi
kazemghaemi@yahoo.com
13700319475328460027367
13700319475328460027367
No
Department of Neurosurgery, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
Mohammad Mehdi
Hassanzadeh-Taheri
mmhtahery35@gmail.com
13700319475328460027368
13700319475328460027368
No
Department of Anatomy, Cellular and Molecular Research Center, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
Mohsen
Foadoddini
foadmohsen@yahoo.com
13700319475328460027369
13700319475328460027369
No
Department of Physiology, Cardiovascular Diseases Research Center, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
Mehran
Hosseini
mehranhosseiny@yahoo.co.in
13700319475328460027370
13700319475328460027370
No
Department of Anatomy, Cellular and Molecular Research Center, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.