Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1398
8
1
gregorian
2019
11
1
10
6
online
1
fulltext
en
Partial Improvement of Spatial Memory Damages by Bone Marrow Mesenchymal Stem Cells Transplantation Following Trimethyltin Chloride Administration in the Rat CA1
Behavioral Neuroscience
Behavioral Neuroscience
Original
Original
<div style="text-align: justify;"><strong>Introduction</strong>: Trimethyltin Chloride (TMT) is a neurotoxin that can kill neurons in the nervous system and activate astrocytes. This neurotoxin mainly damages the hippocampal neurons. After TMT injection, behavioral changes such as aggression and hyperactivity have been reported in animals along with impaired spatial and learning memory. Hence, TMT is a suitable tool for an experimental model of neurodegeneration. The present study aims to determine the palliative effects of Bone Marrow-derived Mesenchymal Stem Cells (BM-MSCs) on the hippocampi of rats damaged from TMT exposure.<br>
<strong>Methods</strong>: We assigned 28 male Wistar rats to the following groups: control, model, vehicle, and treatment. The groups received Intraperitoneal (IP) injections of 8 mg/kg TMT. After one week, stem cells were stereotactically injected into the CA1 of the right rats’ hippocampi. Spatial memory was determined by the Morris Water Maze (MWM) test 6 weeks after cell transplantation. Finally, the rats’ brains were perfused and stained by cresyl violet to determine the numbers of cells in the Cornus Ammonis (CA1) section of the hippocampus. We assessed the expressions of Glial Fibrillary Acidic Protein (GFAP) and Neuronal-specific Nuclear (NeuN) proteins in the right hippocampus by Western blot.<br>
<strong>Results</strong>: The MWM test showed that the treatment group had significantly higher traveled distances in the target quarter compared with the model and vehicle groups (P<0.05). Based on the result of cell count (Nissl staining), the number of cells increased in the treatment group compared with the model and vehicle groups (P<0.05). Western blot results showed up-regulation of GFAP and NeuN proteins in the model, vehicle, and treatment groups compared with the control group. <br>
<strong>Conclusion</strong>: Injection of BM-MSCs may lead to a behavioral and histological improvement in TMT-induced neurotoxicity by increasing the number of pyramidal neurons and improving memory.</div>
Trimethyltin Chloride (TMT), Mesenchymal Stem Cells (MSCs), Hippocampus, Spatial Memory
567
578
http://bcn.iums.ac.ir/browse.php?a_code=A-10-618-5&slc_lang=en&sid=1
Soheila
Madadi
soheila.madadi@yahoo.com
13700319475328460025346
13700319475328460025346
No
Department of Anatomy, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran.
Majid
Katebi
mansourehsoleimani@gmail.com
13700319475328460025347
13700319475328460025347
No
Department of Anatomy, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Mina
Eftekharzadeh
eftekhar.zade@yahoo.com
13700319475328460025348
13700319475328460025348
No
Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Ahmad
Mahdipour
ahmad.mahdipour@yahoo.com
13700319475328460025349
13700319475328460025349
No
Department of Tissue Engineering, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Bagher
Pourheydar
bagherpurheydar@gmail.com
13700319475328460025350
13700319475328460025350
No
Neurophysiology Research Center, Department of Anatomy, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
Mehdi
Mehdizadeh
mehdizadeh.m@iums.ac.ir
13700319475328460025351
13700319475328460025351
Yes
Cellular and Molecular Research Center, Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.