Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1398
2
1
gregorian
2019
5
1
10
3
online
1
fulltext
en
Sex Differences and Role of Gonadal Hormones on Glutamate Level After Spinal Cord Injury in Rats: A Microdialysis Study
Cellular and molecular Neuroscience
Cellular and molecular Neuroscience
Original
Original
<div style="text-align: justify;"><strong>Introduction:</strong> Sex differences in outcomes of Spinal Cord Injury (SCI) suggest a sex-hormone-mediated effect on post-SCI pathological events, including glutamate excitotoxicity. This study aimed to investigate the importance of gonadal hormones on glutamate release subsequent to SCI in rats.<br>
<strong>Methods: </strong>After laminectomy at T8-T9, an electrolytic lesion was applied to the spinothalamic tracts of male and female rats. Using spinal microdialysis, we assessed glutamate levels at the site of lesion in both intact and gonadectomized rats for 4 hours. In this way, we examined the sex differences in the glutamate concentrations.<br>
<strong>Results:</strong> The peak retention time of glutamate level was 10.6 min and spinal glutamate concentration reached a maximum level 40 min following SCI. In male SCI rats, gonadectomy caused a significant elevation of glutamate level (P<0.001) following injury which was maximum 40 min post-SCI as well. However, no significant alterations were seen in gonadectomized female rats.<br>
<strong>Conclusion:</strong> The significant differences in glutamate levels between both intact and gonadectomized SCI male and female rats show the sex-hormone-related mechanisms underlying the molecular events in the second phase of SCI. It seems that the role of male gonadal hormones to prevent glutamate excitotoxicity is more prominent. The exact mechanisms of these hormones on the functional recovery after SCI should be clarified in further studies.</div>
Spinal Cord Injury, Microdialysis, Glutamate, Sex hormones
225
234
http://bcn.iums.ac.ir/browse.php?a_code=A-10-1329-1&slc_lang=en&sid=1
Razieh
Samandari
13700319475328460020550
13700319475328460020550
No
Department of Physiology, Faculty of Basic Sciences, Shahed University, Tehran, Iran.
Majid
Hassanpour-Ezatti
13700319475328460020551
13700319475328460020551
No
Department of Physiology, Faculty of Basic Sciences, Shahed University, Tehran, Iran.
Sajad
Fakhri
Sfakhri@sbmu.ac.ir
13700319475328460020552
13700319475328460020552
No
Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Fatemeh
Abbaszadeh
f.abbaszadehm@gmail.com
13700319475328460020553
13700319475328460020553
No
Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Masoumeh
Jorjani
Msjorjani@sbmu.ac.ir
13700319475328460020554
13700319475328460020554
Yes
Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.